Document Detail


Chemotactic behavior of pathogenic and nonpathogenic Leptospira species.
MedLine Citation:
PMID:  23001652     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B(12) were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.
Authors:
Ambroise Lambert; Naoko Takahashi; Nyles W Charon; Mathieu Picardeau
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-21
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  78     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-15     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8467-9     Citation Subset:  IM    
Affiliation:
Institut Pasteur, Unité de Biologie des Spirochètes, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Load
Chemotactic Factors / analysis
Chemotaxis*
Leptospira / pathogenicity,  physiology*
Real-Time Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
AI29743/AI/NIAID NIH HHS; DE04645/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Chemotactic Factors
Comments/Corrections

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