| Chemosensitivity of conjunctival melanoma cell lines to single chemotherapeutic agents and combinations. | |
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MedLine Citation:
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PMID: 22275346 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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ObjectiveTwo conjunctival cell lines (CRMM-1 and CRMM-2) have been established from recurrent conjunctival melanoma. The authors examined the chemosensitivity of these cell lines to cytotoxic substances and combinations to identify substances that inhibit cell growth efficiently in vitro.Material and methodsCRMM-1 and CRMM-2 were exposed to cisplatin, mitomycin C (MMC), all-trans-retinoic-acid (ATRA), fotemustine or imatinib for 24 h. Sulforhodamine-B assays were used to assess the IC(50). Isobolograms were performed to test possible synergism and antagonism with ATRA or imatinib.ResultsCisplatin and MMC were efficient to inhibit the growth of CRMM-1 and CRMM-2. Combination of imatinib with MMC showed additive antitumoral effect on both cell lines. Combined treatment of imatinib with fotemustine or cisplatin resulted in antagonism. Strong antagonisms were also obtained with ATRA and fotemustine or cisplatin in both cell lines. A synergism was found for ATRA and mitomycin or imatinib in CRMM-2, in contrast to CRMM-1, where antagonism was obtained.ConclusionsCisplatin and MMC inhibit cell growth in conjunctival melanoma cell lines. The potential of ATRA was evident only in combination with MMC or imatinib in CRMM-2 cells. Imatinib and mitomycin increased their efficiency under combination therapy. |
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Authors:
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Henrike Westekemper; Michael Freistuehler; Gerasimos Anastassiou; Gordon Nareyeck; Norbert Bornfeld; Klaus-Peter Steuhl; Max E Scheulen; Ralf A Hilger |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-23 |
Journal Detail:
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Title: The British journal of ophthalmology Volume: - ISSN: 1468-2079 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-25 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421041 Medline TA: Br J Ophthalmol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University Hospital Essen, Essen, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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