Document Detail


Chemoreflex activity increases prostaglandin endoperoxide synthase mRNA expression in the late-gestation fetal sheep brain.
MedLine Citation:
PMID:  21846688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fetal sheep defend blood pressure, blood volume, and blood gases using baro- and chemoreflexes that influence autonomic and neuroendocrine responses. The local generation of prostanoids within the fetal brain is also an important component in activating hormone responses to these stimuli, but the relationship between the reflexes and prostanoid biosynthesis is unclear. The present study was performed to test the hypothesis that the abundances of prostaglandin biosynthetic enzymes in the fetal brain are dependent upon the activity of the baro- and chemoreflex pathways. We subjected chronically catheterized fetal sheep in late gestation to a 10-minute period of brachiocephalic occlusion (BCO), a stimulus that provokes brisk cardiovascular and neuroendocrine responses. We compared the central nervous system abundance of prostaglandin endoperoxide synthases 1 and 2 (PGHS-1 and PGHS-2) after BCO to (1) fetal sheep that had been subjected to BCO after chronic sinoaortic denervation plus bilateral vagotomy and (2) fetal sheep in which the N-methyl d-aspartate (NMDA) receptor antagonist, ketamine, had been administered prior to BCO. Abundances of messenger RNA (mRNA) for PGHS-1 and of mRNA and protein for PGHS-2 in fetal hippocampus were reduced significantly by either prior denervation or ketamine administration. Prostaglandin endoperoxide synthases 1 and 2 mRNA in pituitary were decreased and increased, respectively, by ketamine pretreatment. The results of this study are consistent with the conclusion that the expression of PGHS-1 and -2 in fetal hippocampus and pituitary are influenced by the baro- and/or chemoreflex pathways within the fetal brain in late gestation.
Authors:
Melanie J P Fraites; Charles E Wood
Related Documents :
21315338 - Rupture of endometriotic ovarian cyst causes acute hemoperitoneum in twin pregnancy.
10861718 - Norman-roberts syndrome: prenatal diagnosis and autopsy findings.
2675598 - Comparison of six different ultrasonographic methods for predicting lethal fetal pulmon...
11264098 - The anterior iliac separation: alternative index for pelvic morphometry in fetuses with...
15132198 - Gender in the consulting room.
7944318 - Intraoperative molar pregnancy crisis.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  18     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-17     Completed Date:  2012-01-11     Revised Date:  2013-12-11    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  824-31     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Baroreflex / physiology*
Brachiocephalic Veins / surgery
Brain / embryology*,  enzymology*
Excitatory Amino Acid Antagonists / pharmacology
Female
Isoenzymes
Ketamine / pharmacology
Pregnancy
Prostaglandin-Endoperoxide Synthases / biosynthesis,  genetics*
RNA, Messenger / biosynthesis*,  genetics
Real-Time Polymerase Chain Reaction
Sheep / embryology*
Grant Support
ID/Acronym/Agency:
HD33053/HD/NICHD NIH HHS; R01 HD033053/HD/NICHD NIH HHS; R01 HD033053-11/HD/NICHD NIH HHS; R01 HD033053-12/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Excitatory Amino Acid Antagonists; 0/Isoenzymes; 0/RNA, Messenger; 690G0D6V8H/Ketamine; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Continuous monitoring of pop hole usage by commercially housed free-range hens throughout the produc...
Next Document:  AMG 479, a novel IGF-1-R antibody, inhibits endometrial cancer cell proliferation through disruption...