Document Detail


Chemoradiation interactions under reduced oxygen conditions: Cellular characteristics of an in vitro model.
MedLine Citation:
PMID:  19540039     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Hypoxic tumour regions often contain viable cells that are more resistant to chemotherapy and/or radiotherapy, making it of key importance to analyse new combination treatments under both normoxic and hypoxic conditions. In this study, the impact of moderate hypoxia and anoxia on cellular characteristics was investigated in isogenic A549 cells differing in p53 status. VEGF expression, doubling time, cell cycle distribution, induction of apoptosis and p53 protein expression were evaluated. Radiation survival curves yielded an oxygen enhancement ratio of 1.16-1.67. In conclusion, an in vitro hypoxia model that will be highly useful to analyse chemoradiation interactions is presented.
Authors:
An Wouters; Bea Pauwels; Hilde A J Lambrechts; Greet G O Pattyn; Johan Ides; Marc Baay; Paul Meijnders; Sylvia Dewilde; Jan B Vermorken; Filip Lardon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-18
Journal Detail:
Title:  Cancer letters     Volume:  286     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-17     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  180-8     Citation Subset:  IM    
Affiliation:
Laboratory of Cancer Research and Clinical Oncology, Department of Medical Oncology, University of Antwerp, 2610 Wilrijk, Belgium. An.Wouters@ua.ac.be
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*,  radiation effects
Blotting, Western
Cell Cycle / drug effects,  radiation effects
Cell Hypoxia
Cell Line, Tumor
Cell Proliferation / drug effects,  radiation effects
Cell Survival / drug effects,  radiation effects
Humans
Oxygen / metabolism,  pharmacology*
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Tumor Suppressor Protein p53 / genetics,  metabolism*
Vascular Endothelial Growth Factor A / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Tumor Suppressor Protein p53; 0/Vascular Endothelial Growth Factor A; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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