Document Detail


Chemoprotective effects of curcumin in esophageal epithelial cells exposed to bile acids.
MedLine Citation:
PMID:  20806431     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the ability of curcumin to counteract the impact of bile acids on gene expression of esophageal epithelial cells.
METHODS: An esophageal epithelial cell line (HET-1A) was treated with curcumin in the presence of deoxycholic acid. Cell proliferation and viability assays were used to establish an appropriate dose range for curcumin. The combined and individual effects of curcumin and bile acid on cyclooxygenase-2 (COX-2) and superoxide dismutase (SOD-1 and SOD-2) gene expression were also assessed.
RESULTS: Curcumin in a dose range of 10-100 micromol/L displayed minimal inhibition of HET-1A cell viability. Deoxycholic acid at a concentration of 200 micromol/L caused a 2.4-fold increase in COX-2 gene expression compared to vehicle control. The increased expression of COX-2 induced by deoxycholic acid was partially reversed by the addition of curcumin, and curcumin reduced COX-2 expression 3.3- to 1.3-fold. HET-1A cells exposed to bile acid yielded reduced expression of SOD-1 and SOD-2 genes with the exception that high dose deoxycholic acid at 200 mumol/L led to a 3-fold increase in SOD-2 expression. The addition of curcumin treatment partially reversed the bile acid-induced reduction in SOD-1 expression at all concentrations of curcumin tested.
CONCLUSION: Curcumin reverses bile acid suppression of gene expression of SOD-1. Curcumin is also able to inhibit bile acid induction of COX-2 gene expression.
Authors:
Matthew R Bower; Harini S Aiyer; Yan Li; Robert C G Martin
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  16     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-12-16     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4152-8     Citation Subset:  IM    
Affiliation:
Division of Surgical Oncology, Department of Surgery and James Graham Brown Cancer Center, University of Louisville School of Medicine, 315 East Broadway-Rm 313, Louisville, KY 40202, USA.
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MeSH Terms
Descriptor/Qualifier:
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Bile Acids and Salts / pharmacology*
Cell Line
Cell Proliferation / drug effects
Cell Survival / drug effects
Chemoprevention*
Curcumin / pharmacology*
Cyclooxygenase 2 / genetics,  metabolism
Deoxycholic Acid / pharmacology
Dose-Response Relationship, Drug
Epithelial Cells / cytology,  drug effects*,  metabolism
Esophagus / cytology,  drug effects*,  metabolism
Gene Expression Regulation / drug effects
Humans
Superoxide Dismutase / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
1R03-CA137801/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Bile Acids and Salts; 458-37-7/Curcumin; 83-44-3/Deoxycholic Acid; EC 1.14.99.1/Cyclooxygenase 2; EC 1.15.1.-/superoxide dismutase 1; EC 1.15.1.1/Superoxide Dismutase; EC 1.15.1.1/superoxide dismutase 2
Comments/Corrections

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