Document Detail


Chemopreventive effect of protein extract of Asterina pectinifera in HT-29 human colon adenocarcinoma cells.
MedLine Citation:
PMID:  16596993     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the effect of protein extract of Asterina pectinifera on the activity of 4 enzymes that may play a role in adenocarcinoma of the colon: quinone reductase (QR), glutathione S-transferase (GST), ornithine decarboxylase (ODC), and cyclooxygenase (COX)-2. QR and GST activity increased in HT-29 human colon adenocarcinoma cells increased that had been exposed to 4 concentrations of the protein extract (80, 160, 200, and 240 microg/mL). Additionally, 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ODC activity decreased significantly in cells exposed to the extract in concentrations of 160 microg/mL (p<0.05), 200 microg/mL (p<0.005), and 240 microg/mL (p<0.005). TPA-induced COX-2 activity also decreased in cells exposed to extract concentrations of 10, 20, 40, and 60 microg/mL. COX-2 expression was also inhibited in cells exposed to this extract. These results suggest that this protein extract of A pectinifera has chemopreventive activity in HT-29 human colon adenocarcinoma cells, and therefore, may have the potential to function as a chemopreventive agent in human colorectal cancer.
Authors:
Yun-Hee Shon; Kyung-Soo Nam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of pharmacal research     Volume:  29     ISSN:  0253-6269     ISO Abbreviation:  Arch. Pharm. Res.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-04-06     Completed Date:  2006-07-17     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8000036     Medline TA:  Arch Pharm Res     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  209-12     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University, Gyeongju, Korea.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma
Antineoplastic Agents / isolation & purification,  pharmacology*
Asterina* / chemistry
Colonic Neoplasms
Cyclooxygenase 2 / metabolism
Cyclooxygenase 2 Inhibitors / isolation & purification,  pharmacology*
Dose-Response Relationship, Drug
Glutathione Transferase / metabolism
HT29 Cells / drug effects*,  enzymology
Humans
NAD(P)H Dehydrogenase (Quinone) / metabolism
Ornithine Decarboxylase / antagonists & inhibitors,  metabolism
Proteins / isolation & purification,  pharmacology*
Tetradecanoylphorbol Acetate
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Cyclooxygenase 2 Inhibitors; 0/Proteins; 16561-29-8/Tetradecanoylphorbol Acetate; EC 1.14.99.1/Cyclooxygenase 2; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 2.5.1.18/Glutathione Transferase; EC 4.1.1.17/Ornithine Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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