| Chemopreventive effect of epigallocatechin-3-gallate (EGCG) and folic acid on the N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastrointestinal cancer in rat model. | |
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MedLine Citation:
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PMID: 21615871 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the chemopreventive effect and mechanisms of epigallocatechin-3-gallate (EGCG) and folic acid on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastrointestinal cancer in rats, and to investigate and compare the combinatorial effects of EGCG and folic acid on the chemoprevention of gastrointestinal carcinogenesis. METHODS: A total of 159 healthy male Wistar rats were randomly divided into seven groups to have the MNNG in drink (group M), MNNG in drink and EGCG in the feed (group ME), MNNG in drink and folic acid in the feed (group MF), MNNG in drink and EGCG + folic acid in the feed (group MEF), EGCG in the feed (group E), folic acid in the feed (group F) or normal feed (group C), respectively. At 44 weeks, all the rats were killed and assessed for the presence of gastrointestinal tumor. The occurrence of cancer was evaluated by histology. Ki-67 in cancerous tissues and in situ apoptosis were determined by immunohistochemical staining or terminal deoxyribonucleotide transferase-mediated nick-end labeling (TUNEL) assay, respectively. RESULTS: The experiment was completed in 157 rats (98.74%). As compared with group M, the tumor incidence of group MEF decreased significantly (P = 0.011). Ki-67 expression in cancerous tissues of group ME and MEF also decreased significantly (P = 0.038, P = 0.009), while apoptosis of group ME, MF and MEF increased significantly (P = 0.000, P = 0.003, P = 0.000). CONCLUSION: EGCG combined with folic acid has an obvious chemopreventive effect on gastrointestinal carcinogenesis induced by MNNG in rats. |
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Authors:
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Qi Xu; Chuan Hua Yang; Qiang Liu; Xi Feng Jin; Xi Tao Xu; Jin Lu Tong; Shu Dong Xiao; Zhi Hua Ran |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of digestive diseases Volume: 12 ISSN: 1751-2980 ISO Abbreviation: J Dig Dis Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-05-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101302699 Medline TA: J Dig Dis Country: Australia |
Other Details:
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Languages: eng Pagination: 181-7 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. Journal of Digestive Diseases © 2011 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Blackwell Publishing Asia Pty Ltd. |
Affiliation:
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Department of Gastroenterology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Disease, Shanghai, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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