Document Detail

Chemopreventive activity of polyphenolics from black Jamapa bean (Phaseolus vulgaris L.) on HeLa and HaCaT cells.
MedLine Citation:
PMID:  16536584     Owner:  NLM     Status:  MEDLINE    
The antiproliferative effects of 100% methanol crude extract and of Toyopearl and silica gel fractions from the seed coats of black Jamapa beans (Phaseolus vulgaris L.) were evaluated using HeLa, human adenocarcinoma cells, and HaCaT, human premalignant keratinocytes. The 100% methanol crude extract [172.2 microM equiv of (+)-catechin] increased adhesion of HeLa cells; however, 3- and 5-fold higher concentrations decreased the number of cells attached as a function of the treatment time. The highest concentration tested diminished the cell adhesion until 40% (after 24 h) to almost 80% (after 72 h). The IC50 values showed that the 100% methanol crude extract was the most effective inhibitor of HeLa cell proliferation, even when it was dissolved in dimethylsulfoxide (DMSO) [34.5 microM equiv of (+)-catechin] or in medium [97.7 microM equiv of (+)-catechin]. The Toyopearl 5 (TP5) fraction and silica gel 2 (SG2) fraction inhibited 60% of the HeLa cell proliferation. The IC50 was 154 microM equiv of (+)-catechin of the 100% methanol crude extract on HaCaT cells. Toyopearl fractions TP4 and TP6 significantly inhibited HaCaT cell proliferation, but the silica gel fractions did not have a significant effect. The 100% methanol crude extract (35 microg of dry material/mL) decreased the number of HeLa cells in the G0/G1 phase from 68.9% (for control cells) to 51.4% (for treated cells) and increased apoptosis (2.9 and 21.2% for control and treated cells, respectively). The results indicated that black Jamapa beans could be a source of polyphenolic compounds, which have an inhibitory effect toward HeLa cancer cells but are less aggressive on HaCaT premalignant cells.
Xochitl Aparicio-Fernández; Teresa García-Gasca; Gad G Yousef; Mary Ann Lila; Elvira González de Mejia; Guadalupe Loarca-Pina
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-15     Completed Date:  2006-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2116-22     Citation Subset:  IM    
Programa de Posgrado en Alimentos del Centro de la Republica, Research and Graduate Studies in Food Science, School of Chemistry, Universidad Autónoma de Querétaro, Querétaro, Qro., 76010 México.
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MeSH Terms
Anticarcinogenic Agents / pharmacology*
Apoptosis / drug effects
Cell Adhesion / drug effects
Cell Cycle / drug effects
Cell Division / drug effects
Cell Line, Tumor
Flavonoids / pharmacology*
Hela Cells
Phaseolus / chemistry*
Phenols / pharmacology*
Plant Extracts / pharmacology
Precancerous Conditions
Seeds / chemistry*
Skin Neoplasms
Reg. No./Substance:
0/Anticarcinogenic Agents; 0/Flavonoids; 0/Phenols; 0/Plant Extracts; 0/polyphenols; 67-56-1/Methanol

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