Document Detail


Chemoprevention by white currant is mediated by the reduction of nuclear beta-catenin and NF-kappaB levels in Min mice adenomas.
MedLine Citation:
PMID:  18389329     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Berries are a good natural source of phenolic compounds and many berries or their compounds have been shown to be chemopreventive. White currant is an interesting berry, as it contains low levels of dominant berry phenolics such as ellagic acid, anthocyanins and other flavonoids. AIMS OF THE STUDY: To study if white currant is chemopreventive in an experimental model for intestinal tumorigenesis and further study the effects on beta-catenin and NF-kappaB signaling pathways. METHODS: Multiple intestinal neoplasia (Min) mice were fed an AIN-93G based control diet or a diet containing 10% freeze dried white currant (Ribes x pallidum) for 10 weeks. Cell signaling parameters were analysed from intestinal adenomas and surrounding mucosa by Western blotting and immunohistochemistry. RESULTS: The white currant diet reduced the number of adenomas from 81 (min-max 47-114) to 51 (36-84) in the total small intestine of Min mice (P<0.02). Most of the adenomas develop in the distal part of the small intestine, and in this area white currant reduced the number from 49 to 29.5 (P<0.01) and also the size of the adenomas from 0.88 mm to 0.70 mm (P<0.02). In the colon white currant increased the number of adenomas (0.3+/-0.6 vs. 0.8+/-0.6, mean +/- SD, P<0.05), but did not affect the size. White currant reduced nuclear beta-catenin and NF-kappaB protein levels in the adenomas (P<0.05 and P<0.02, respectively). They were correlated with the size of adenomas (P<0.01). CONCLUSIONS: This study shows that white currant is effective in preventing cancer initiation and progression in the Min mouse. Whether the positive effects are due to its special phenolic composition needs to be studied in more detail.
Authors:
Johanna Rajakangas; Marjo Misikangas; Essi Päivärinta; Marja Mutanen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-03
Journal Detail:
Title:  European journal of nutrition     Volume:  47     ISSN:  1436-6207     ISO Abbreviation:  Eur J Nutr     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-05-15     Completed Date:  2008-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888704     Medline TA:  Eur J Nutr     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  115-22     Citation Subset:  IM    
Affiliation:
Dept. of Applied Chemistry and Microbiology (Nutrition), University of Helsinki, P.O. Box 66, 00014 Helsinki, Finland. johanna.rajakangas@helsinki.fi
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MeSH Terms
Descriptor/Qualifier:
Adenoma / genetics,  prevention & control*
Animals
Antineoplastic Agents, Phytogenic / pharmacology*
Female
Fruit / chemistry*
Intestinal Mucosa / pathology*
Intestinal Neoplasms / genetics,  prevention & control*
Male
Mice
Mice, Mutant Strains
NF-kappa B / metabolism*
Random Allocation
Signal Transduction
beta Catenin / metabolism*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/NF-kappa B; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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