| Chemokines regulate small leucine-rich proteoglycans in the extracellular matrix of the pressure-overloaded right ventricle. | |
| | |
MedLine Citation:
|
PMID: 22345433 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Chemokines have been suggested to play a role during development of left ventricular failure, but little is known about their role during right ventricular (RV) remodeling and dysfunction. We have previously shown that the chemokine CXCL13 regulates small leucine-rich proteoglycans (SLRPs). We hypothesized that chemokines are upregulated in the pressure-overloaded RV and that they regulate SLRPs. Mice with RV pressure-overload following pulmonary banding (PB) had a significant increase in RV weight and an increase in liver weight after one week. Microarray analysis (Affymetrix) of RV tissue from mice with PB revealed that CXCL10, CXCL6, CX3CL1, CCL5, CXCL16 and CCL2 were the most upregulated chemokines. Stimulation of cardiac fibroblasts with these same chemokines showed that CXCL16 increased the expression of the four SLRPs decorin, lumican, biglycan and fibromodulin. CCL5 increased the same SLRPs except decorin, while CX3CL1 increased the expression of decorin and lumican. CXCL16, CX3CL1 and CCL5 were also shown to increase the levels of glycosylated decorin and lumican in the medium after stimulation of fibroblasts. In the pressure-overloaded RV tissue, Western blotting revealed an increase in the total protein level of lumican and a glycosylated form of decorin with a higher molecular weight compared to control mice. Both mice with PB and patients with pulmonary stenosis had significantly increased circulating levels of CXCL16 compared to healthy controls measured by enzyme immunoassay. In conclusion, we have found that chemokines are upregulated in the pressure-overloaded RV and that CXCL16, CX3CL1 and CCL5 regulate expression and post-translational modifications of SLRPs in cardiac fibroblasts. In the pressure-overloaded RV, protein levels of lumican were increased, and a glycosylated form of decorin with a high molecular weight appeared. |
| | |
Authors:
|
Anne Wæhre; Maria Vistnes; Ivar Sjaastad; Ståle Nygård; Cathrine Husberg; Ida Gjervold Lunde; Pål Aukrust; Arne Yndestad; Leif Erik Vinge; Dina Behmen; Christian Neukamm; Henrik Brun; Erik Thaulow; Geir Christensen |
Related Documents
:
|
8155443 - Haemodynamics of liver reperfusion: comparison of two anaesthetic techniques. 385143 - A computerised dichromatic earpiece densitometer for the measurement of cardiac output. 2240793 - Effect of endotoxin administration on equine digital hemodynamics and starling forces. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-2-16 |
Journal Detail:
|
Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: - Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-2-20 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1Oslo University Hospital, Ullevål. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of lactate on the voltage-gated sodium channels of rat skeletal muscle: modulating current o...
Next Document: The Effect of Rowing Ergometry and Oral Volume Loading on Cardiovascular Structure and Function Duri...