Document Detail

Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors.
MedLine Citation:
PMID:  20100199     Owner:  NLM     Status:  MEDLINE    
Keratinocytes in the skin play an important role in innate immune responses by secreting chemokines. This study aimed to determine if keratinocyte cell lines can be used for studies of innate immune mechanisms. Human primary keratinocytes and the HaCaT, CCD 1106 KERTr (KERTr) and HEK001 cell lines were treated with a panel of Toll-like receptor (TLR)-ligands. Expression of IL-8, CCL20, CXCL9 and CXCL10 was determined. All three cell lines expressed TLR1-6 and TLR9. KERTr cells responded to the same TLR-ligands as primary keratinocytes. Overall HEK001 responded similarly, but appeared to be relatively more sensitive to flagellin. This was in agreement with increased expression of TLR5. The expression profiles were most distinct in HaCaT cells. Furthermore, our data confirm and extend previously reported TLR7 and TLR8 independent IL-8 secretion by keratinocytes after Imiquimod treatment. The different cell lines represent complementary tools for molecular studies of innate immunity of the skin.
Florina Olaru; Liselotte E Jensen
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Publication Detail:
Type:  Letter; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  19     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2011-01-06     Revised Date:  2014-09-21    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  e314-6     Citation Subset:  IM    
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MeSH Terms
Cell Line
Chemokines / metabolism*
Immunity, Innate / physiology
Keratinocytes / cytology,  metabolism*
Toll-Like Receptor 5 / metabolism
Toll-Like Receptor 6 / metabolism
Toll-Like Receptor 9 / metabolism
Toll-Like Receptors / metabolism*
Grant Support
Reg. No./Substance:
0/Chemokines; 0/Ligands; 0/TLR5 protein, human; 0/TLR6 protein, human; 0/TLR9 protein, human; 0/Toll-Like Receptor 5; 0/Toll-Like Receptor 6; 0/Toll-Like Receptor 9; 0/Toll-Like Receptors

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