Document Detail


Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors.
MedLine Citation:
PMID:  20100199     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Keratinocytes in the skin play an important role in innate immune responses by secreting chemokines. This study aimed to determine if keratinocyte cell lines can be used for studies of innate immune mechanisms. Human primary keratinocytes and the HaCaT, CCD 1106 KERTr (KERTr) and HEK001 cell lines were treated with a panel of Toll-like receptor (TLR)-ligands. Expression of IL-8, CCL20, CXCL9 and CXCL10 was determined. All three cell lines expressed TLR1-6 and TLR9. KERTr cells responded to the same TLR-ligands as primary keratinocytes. Overall HEK001 responded similarly, but appeared to be relatively more sensitive to flagellin. This was in agreement with increased expression of TLR5. The expression profiles were most distinct in HaCaT cells. Furthermore, our data confirm and extend previously reported TLR7 and TLR8 independent IL-8 secretion by keratinocytes after Imiquimod treatment. The different cell lines represent complementary tools for molecular studies of innate immunity of the skin.
Authors:
Florina Olaru; Liselotte E Jensen
Related Documents :
19805319 - Metalloproteinase mt5-mmp is an essential modulator of neuro-immune interactions in the...
16879839 - Cell type and dna damage specific response of human skin cells to environmental agents.
7681089 - Expression of distinct desmocollin isoforms in human epidermis.
19263059 - The serotonin transporter protein is expressed in psoriasis, where it may play a role i...
15788149 - Pimecrolimus--a safe and effective local immunomodulator in the treatment of inflammato...
21402899 - Ifn-α mediates the development of autoimmunity both by direct tissue toxicity and thro...
17603689 - The role of apoptosis in autoantibody production.
18642699 - Inversion of stress response reprogramming phenomenon in lipopolysaccharide-stimulated ...
15806279 - Decreased production of nitric oxide by peripheral blood mononuclear cells of patients ...
Publication Detail:
Type:  Letter; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental dermatology     Volume:  19     ISSN:  1600-0625     ISO Abbreviation:  Exp. Dermatol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2011-01-06     Revised Date:  2013-04-09    
Medline Journal Info:
Nlm Unique ID:  9301549     Medline TA:  Exp Dermatol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  e314-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line
Chemokines / metabolism*
Humans
Immunity, Innate / physiology
Keratinocytes / cytology,  metabolism*
Ligands
Toll-Like Receptor 5 / metabolism
Toll-Like Receptor 6 / metabolism
Toll-Like Receptor 9 / metabolism
Toll-Like Receptors / metabolism*
Grant Support
ID/Acronym/Agency:
AR053672/AR/NIAMS NIH HHS; R03 AR053672/AR/NIAMS NIH HHS; R03 AR053672-03/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Chemokines; 0/Ligands; 0/TLR5 protein, human; 0/TLR6 protein, human; 0/TLR9 protein, human; 0/Toll-Like Receptor 5; 0/Toll-Like Receptor 6; 0/Toll-Like Receptor 9; 0/Toll-Like Receptors
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Differential expression of antimicrobial peptides in margins of chronic wounds.
Next Document:  Enhanced MCP-1 release by keloid CD14+ cells augments fibroblast proliferation: role of MCP-1 and Ak...