| Chemokine expression by human keratinocyte cell lines after activation of Toll-like receptors. | |
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MedLine Citation:
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PMID: 20100199 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Keratinocytes in the skin play an important role in innate immune responses by secreting chemokines. This study aimed to determine if keratinocyte cell lines can be used for studies of innate immune mechanisms. Human primary keratinocytes and the HaCaT, CCD 1106 KERTr (KERTr) and HEK001 cell lines were treated with a panel of Toll-like receptor (TLR)-ligands. Expression of IL-8, CCL20, CXCL9 and CXCL10 was determined. All three cell lines expressed TLR1-6 and TLR9. KERTr cells responded to the same TLR-ligands as primary keratinocytes. Overall HEK001 responded similarly, but appeared to be relatively more sensitive to flagellin. This was in agreement with increased expression of TLR5. The expression profiles were most distinct in HaCaT cells. Furthermore, our data confirm and extend previously reported TLR7 and TLR8 independent IL-8 secretion by keratinocytes after Imiquimod treatment. The different cell lines represent complementary tools for molecular studies of innate immunity of the skin. |
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Authors:
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Florina Olaru; Liselotte E Jensen |
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Publication Detail:
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Type: Letter; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental dermatology Volume: 19 ISSN: 1600-0625 ISO Abbreviation: Exp. Dermatol. Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2011-01-06 Revised Date: 2013-04-09 |
Medline Journal Info:
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Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: Denmark |
Other Details:
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Languages: eng Pagination: e314-6 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Chemokines / metabolism* Humans Immunity, Innate / physiology Keratinocytes / cytology, metabolism* Ligands Toll-Like Receptor 5 / metabolism Toll-Like Receptor 6 / metabolism Toll-Like Receptor 9 / metabolism Toll-Like Receptors / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AR053672/AR/NIAMS NIH HHS; R03 AR053672/AR/NIAMS NIH HHS; R03 AR053672-03/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokines; 0/Ligands; 0/TLR5 protein, human; 0/TLR6 protein, human; 0/TLR9 protein, human; 0/Toll-Like Receptor 5; 0/Toll-Like Receptor 6; 0/Toll-Like Receptor 9; 0/Toll-Like Receptors |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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