Document Detail


Chemokine programming dendritic cell antigen response: part I - select chemokine programming of antigen uptake even after maturation.
MedLine Citation:
PMID:  23278719     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Here, we report on the successful programming of dendritic cells (DCs) using selectively applied mixtures of chemokines as a novel protocol for engineering vaccine efficiency. Antigen internalization by DCs is a pivotal step in antigen uptake/presentation for bridging innate and adaptive immunity and in exogenous gene delivery used in vaccine strategies. Contrary to most approaches to improve vaccine efficiency, active enhancement of antigen internalization by DCs as a vaccine strategy has been less studied because DCs naturally down-regulate antigen internalization upon maturation. Whereas chemokines are mainly known as signal proteins that induce leucocyte chemotaxis, very little research has been carried out to identify any additional effects of chemokines on DCs following maturation. Here, immature DCs are pre-treated with select chemokines before intentional maturation using lipopolysaccharide (LPS). When pre-treated with a mixture of CCL3 and CCL19 in a 7 : 3 ratio, then matured with LPS, chemokine pre-treated DCs exhibited 36% higher antigen uptake capacity than immature DCs and 27% higher antigen-processing capacity than immature DCs treated only with LPS. Further, CCL3 : CCL19 (7 : 3) pre-treatment of DCs modulated MHC molecule expression and secretion of various cytokines of DCs. Collectively, DC programming was feasible using a specific chemokine combination and these results provide a novel strategy for enhancing DC-based vaccine efficiency. In Part II, we report on the phenotype changes and antigen presentation capacity of chemokine pre-treated murine bone marrow-derived DCs examined in long-term co-culture with antigen-specific CD4(+) T cells.
Authors:
Jaehyung Park; Cindy T Wu; James D Bryers
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Immunology     Volume:  139     ISSN:  1365-2567     ISO Abbreviation:  Immunology     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-09     Completed Date:  2013-06-07     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  0374672     Medline TA:  Immunology     Country:  England    
Other Details:
Languages:  eng     Pagination:  72-87     Citation Subset:  IM    
Copyright Information:
© 2012 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation / drug effects,  immunology*
Cell Line
Chemokine CCL19 / immunology*,  pharmacology
Chemokine CCL3 / immunology*,  pharmacology
Dendritic Cells / immunology*
Mice
Vaccines / immunology
Grant Support
ID/Acronym/Agency:
R01 AI074661/AI/NIAID NIH HHS; R01 DE018701/DE/NIDCR NIH HHS; R01AI074661/AI/NIAID NIH HHS; R01DE018701/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Ccl19 protein, mouse; 0/Ccl3 protein, mouse; 0/Chemokine CCL19; 0/Chemokine CCL3; 0/Vaccines
Comments/Corrections

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