| Chemiluminescence receptor assay for measuring vitamin B12 in serum evaluated. | |
| | |
MedLine Citation:
|
PMID: 8149606 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We evaluated a chemiluminescence receptor assay for vitamin B12 in serum (Magic Lite; Ciba Corning Diagnostics), in which an acridinium ester label is used with magnetic particle separation. Within- and between-batch precisions were generally acceptable, except at low analyte concentrations. The reference range determined from 104 elective preoperative patients was 120-610 pmol/L, compared with 150-590 pmol/L for our in-house radioligand-binding assay. Magic Lite discriminated between normal and abnormal results as effectively as the in-house method when local reference ranges were applied. Magic Lite demonstrated a negative bias at low analyte concentrations and was unable to detect any vitamin B12 in two B12-deficient patients. Assay accuracy--judged from analytical recovery and comparisons with the in-house method and two other radioassay kits (Quantaphase, Bio-Rad Labs., and Immophase, Ciba Corning Diagnostics)--was poor at low B12 concentrations when the manufacturer's recommended two-point calibration was used. This problem was partially corrected by using a full set of calibrators. |
| | |
Authors:
|
S Wentworth; J A McBride; W H Walker |
Related Documents
:
|
3192936 - Analysis of commercial samples of acridine orange using high-performance liquid chromat... 578146 - Reverse phase high pressure liquid chromatography of vitamin a in margarine, infant for... 7118786 - Application of gel permeation chromatography and nonaqueous reverse phase chromatograph... 11472896 - Multicenter collaborative study to calibrate salmon calcitonin by bioassay and high-per... 12668086 - Optimization of a solid-phase extraction method for determination of indapamide in biol... 8686886 - Capillary electrophoretic enzyme immunoassay for digoxin in human serum. |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Clinical chemistry Volume: 40 ISSN: 0009-9147 ISO Abbreviation: Clin. Chem. Publication Date: 1994 Apr |
Date Detail:
|
Created Date: 1994-05-06 Completed Date: 1994-05-06 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 537-40 Citation Subset: IM |
Affiliation:
|
Department of Laboratory Medicine, Henderson General Hospital, Hamilton, Ontario, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Chemiluminescent Measurements* Humans Kinetics Magnetics Microspheres Quality Control Radioligand Assay / methods*, statistics & numerical data Reagent Kits, Diagnostic / statistics & numerical data Reference Values Sensitivity and Specificity Vitamin B 12 / blood* |
| Chemical | |
Reg. No./Substance:
|
0/Reagent Kits, Diagnostic; 68-19-9/Vitamin B 12 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Early detection of metabolic abnormalities in preterm infants impaired by disorders of blood glucose...
Next Document: Diagnostic power of lecithin/sphingomyelin ratio and fluorescence polarization assays for respirator...