Document Detail


Chemiluminescence microflow injection analysis system on a chip for the determination of uric acid without enzyme.
MedLine Citation:
PMID:  16134228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new microflow injection analysis (microFIA) system on a chip coupled with chemiluminescence (CL) for the non-enzymatic determination of uric acid is described. The microFIA system produced by using two transparent poly(methylmethacrylate) (PMMA) chips measured 50 x 40 x 5 mm, the microchannels, etched by CO2 laser, were 200 microm wide and 100 microm deep, and the volume of the reaction area (RA) was about 1.2 microL. The injection pump, with accurate time control, monitored all reagents, including the sample. The uric acid was sensed by the chemiluminescence reaction between luminol and ferricyanide. The linear range of the uric acid concentration was 0.8-30 mg/L and the detection limit was 0.5 mg/L (S/N = 3). The relative standard deviation was 4.42% for 5 mg/L uric acid (n = 8). The proposed method has been successfully applied to the non-separation determination of uric acid in human serum and urine.
Authors:
Deyong He; Zhujun Zhang; Ying Huang; Yufei Hu; Houjiang Zhou; Dinglong Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  20     ISSN:  1522-7235     ISO Abbreviation:  Luminescence     Publication Date:    2005 Jul-Oct
Date Detail:
Created Date:  2005-09-12     Completed Date:  2005-12-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  England    
Other Details:
Languages:  eng     Pagination:  271-5     Citation Subset:  IM    
Copyright Information:
Copyright 2005 John Wiley & Sons, Ltd.
Affiliation:
Institute of Analytical Science, the Key Laboratory of Analytical Chemistry of Chongqing, Southwest Normal University, Beibei, Chongqing 400715, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Chemiluminescent Measurements / instrumentation*,  methods*
Ferricyanides / pharmacology
Flow Injection Analysis / methods*
Humans
Luminol / pharmacology
Sodium Hydroxide / pharmacology
Uric Acid / blood*,  chemistry,  urine*
Chemical
Reg. No./Substance:
0/Ferricyanides; 1310-73-2/Sodium Hydroxide; 13746-66-2/potassium ferricyanide; 521-31-3/Luminol; 69-93-2/Uric Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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