| Chemiluminescence determination of cefotaxime sodium with flow-injection analysis of cerium (IV)-rhodamine 6G system and its application to the binding study of cefotaxime sodium to protein with on-line microdialysis sampling. | |
| | |
MedLine Citation:
|
PMID: 21050805 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
A simple, sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of cefotaxime sodium based on the chemiluminescence reaction of cefotaxime sodium with ceric sulfate and rhodamine 6G in nitric acid solution. The concentration of cefotaxime sodium was proportional with the CL intensity in the range of 4×10(-8)-8×10(-6) mol L(-1). The detection limit (signal-to-noise ratio=3) was 1×10(-8) mol L(-1). Coupled to the technique of on-line microdialysis sampling, this method was successfully applied to study cefotaxime sodium-protein interaction. The drug and protein were mixed in different molar ratios in Ringer's solution, pH 7.4, and incubated at 37°C in a water bath. The microdialysis probe was utilized to sample the mixed solution at a perfusion rate of 5 μL min(-1) and the recovery of cefotaxime sodium under experimental condition was 16.2%. The data obtained by the present Microdialysis-Flow Injection Analysis-CL method was analyzed with the Scatchard analysis and Klotz plot. The estimated association constant (K) and the number of the binding sites (n) on one of BSA molecule were 5.94×10(4) M(-1) and 1.29 (Klotz equation), respectively. |
| | |
Authors:
|
Dinglong Chen; Huiying Wang; Zhujun Zhang; Lijie Ci; Xuehong Zhang |
Related Documents
:
|
18969055 - Chromatographic determinations of a beta-lactam antibiotic, cefaclor by means of fluore... 17386615 - Determination of propoxur in environmental samples by automated solid-phase extraction ... 18558775 - Sampling-resolution strategy for one-way multiplexed immunoassay with sequential chemil... 2093385 - Chemiluminescence: a sensitive and versatile method for analyte detection. 21941035 - A porous silicon optical microcavity for sensitive bacteria detection. 16134195 - A rapid capillary electrophoresis with electrochemiluminescence method for the assay of... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-03 |
Journal Detail:
|
Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 78 ISSN: 1873-3557 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-27 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
|
Languages: eng Pagination: 553-7 Citation Subset: IM |
Copyright Information:
|
Copyright © 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
College of Chemical Engineering, Shijiazhuang University, Shijiazhuang 050035, PR China. chendl1230@126.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: FtsZ-less cell division in archaea and bacteria.
Next Document: Raman spectra of biomarkers of relevance to analytical astrobiological exploration: hopanoids, stero...