| Chemiluminescence and chemotaxis assay of canine granulocytes: a methodological study. | |
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MedLine Citation:
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PMID: 2399874 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Chemiluminescence (CL) of isolated granulocytes and of whole blood from dogs was evaluated. Chemiluminescence of whole blood samples created an undesired quenching effect by the red blood cells which makes the assay difficult to apply in pathological cases with low formation of oxygen metabolites. This problem was avoided when chemiluminescence was determined, using isolated granulocytes. A cell concentration of 5 x 10(9)/l was needed to create optimal conditions. The Boyden chamber technique was used for study of random migration and chemotaxis. Casein (0.1%), zymosan activated serum with and without epsilon-amino-n-caproic-acid and homologous serum were effective chemoattractants for canine granulocytes, while FMLP (formyl-methionyl-leucyl-phenylalanin) did not attract canine granulocytes. |
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Authors:
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G Trowald-Wigh; K Thorén-Tolling |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta veterinaria Scandinavica Volume: 31 ISSN: 0044-605X ISO Abbreviation: Acta Vet. Scand. Publication Date: 1990 |
Date Detail:
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Created Date: 1990-10-15 Completed Date: 1990-10-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370400 Medline TA: Acta Vet Scand Country: DENMARK |
Other Details:
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Languages: eng Pagination: 79-86 Citation Subset: IM |
Affiliation:
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Department of Medicine and Surgery, Veterinary Faculty, Swedish University of Agricultural Sciences, Uppsala, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemotaxis, Leukocyte* Dogs / blood* Female Granulocytes / immunology* Luminescent Measurements Male |
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