Document Detail


Chemiluminescence assay for uric acid in human serum and urine using flow-injection with immobilized reagents technology.
MedLine Citation:
PMID:  11936107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel chemiluminescence (CL) flow sensor for the determination of uric acid in human urine and serum has been developed by using controlled-reagent-release technology. The reagents involved in the chemiluminescence (CL) reaction, luminol and periodate, are immobilized on anion-exchange resin packed in a column. After injection of water, chemiluminescence generated by released luminol and periodate in alkaline media is inhibited in presence of uric acid. By measuring the decreased chemiluminescence (CL) intensity the uric acid is sensed. The decreased response is linear in the 5.0-500.0 ng mL(-1) range, with a detection limit of 1.8 ng mL(-1). The flow sensor showed remarkable operational stability and could be easily reused for over 80 h with sampling frequency of 100 h(-1). The proposed sensor was applied to the determination of uric acid in human urine and serum, and monitoring metabolic uric acid in human urine with RSD less than 3.0%.
Authors:
Zheng-Hua Song; Shuang Hou
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Publication Detail:
Type:  Journal Article     Date:  2001-12-20
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  372     ISSN:  1618-2642     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-04-08     Completed Date:  2003-03-21     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  327-32     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Northwest University, Xi'an, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / methods*,  standards
Calibration
Chemiluminescent Measurements
Equipment Reuse
Humans
Hydrogen-Ion Concentration
Indicators and Reagents
Ion Exchange Resins
Luminol
Periodic Acid
Sensitivity and Specificity
Uric Acid / blood*,  urine*
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Ion Exchange Resins; 10450-60-9/Periodic Acid; 521-31-3/Luminol; 69-93-2/Uric Acid; 7790-28-5/metaperiodate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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