| Chemiluminescence Response of Murine Macrophages on Multilayer Microfluidic Chips. | |
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MedLine Citation:
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PMID: 22139733 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have demonstrated an integrated platform for microfluidics and chemiluminescence (CL) detection that is capable of parallel cell culture, convenient liquid manipulation, and sensitive chemiluminescent detection. Luminol-dependent CL responses induced by three different stimuli, phytohemagglutinin (PHA), concanavalin A (ConA), and lipopolysaccharides (LPS), which can evoke a CL response in macrophages, were evaluated on this microfluidic chip. We studied the dose-dependent effect of these three stimuli on CL response in murine macrophages. PHA produced the highest CL response compared to LPS and ConA. The CL intensity produced by PHA was 6.85 and four times higher than that by LPS and ConA, respectively, at the low concentration of 100 μg/ml. We have found microfluidic based CL to be a very useful screening tool, which is less laborious and more sensitive. This microfluidic system is disposable and capable of rapid device prototyping; it may prove to be very useful in clinical and pharmaceutical applications. |
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Authors:
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Linfen Yu; Qun Li; Hongwei Gai; Zhanhui Wang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-3 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Research Center of Biosensors and Medical Instruments, Shenzhen Institutes of Advanced Technology, CAS, Shenzhen, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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