Document Detail


Chemically specific imaging and in-situ chemical analysis of articular cartilage with stimulated Raman scattering.
MedLine Citation:
PMID:  23303610     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Stimulated Raman scattering (SRS) has been applied to unstained samples of articular cartilage enabling the investigation of living cells within fresh tissue. Hyperspectral SRS measurements over the CH vibrational region showed variations in protein and lipid content within the cells, pericellular matrix and interterritorial matrix. Changes in the cells and pericellular matrix were investigated as a function of depth into the cartilage. Lipid was detected in the pericellular matrix of superficial zone chondrocytes. The spectral profile of lipid droplets within the chondrocytes indicated that they contained predominantly unsaturated lipids. The mineral content has been imaged by using the $ {\rm PO^{3-}_{4} $ vibration at 959 cm(-1) and the $ {\rm CO^{2-}_{3} $ vibration at 1070 cm(-1) . Both changes in cells and mineralization are known to be important factors in the progression of osteoarthritis. SRS enables these to be visualized in fresh unstained tissue and consequently should benefit osteoarthiritis research. (© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
Authors:
Jessica Mansfield; Julian Moger; Ellen Green; Charlotte Moger; C Peter Winlove
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Journal of biophotonics     Volume:  -     ISSN:  1864-0648     ISO Abbreviation:  J Biophotonics     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101318567     Medline TA:  J Biophotonics     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Affiliation:
Physics, University of Exeter, Stocker Rd, Exeter, EX4 4QL, UK. j.c.mansfield@exeter.ac.uk.
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