Document Detail

Chemically resolved imaging of biological cells and thin films by infrared scanning near-field optical microscopy.
MedLine Citation:
PMID:  14507733     Owner:  NLM     Status:  MEDLINE    
The infrared (IR) absorption of a biological system can potentially report on fundamentally important microchemical properties. For example, molecular IR profiles are known to change during increases in metabolic flux, protein phosphorylation, or proteolytic cleavage. However, practical implementation of intracellular IR imaging has been problematic because the diffraction limit of conventional infrared microscopy results in low spatial resolution. We have overcome this limitation by using an IR spectroscopic version of scanning near-field optical microscopy (SNOM), in conjunction with a tunable free-electron laser source. The results presented here clearly reveal different chemical constituents in thin films and biological cells. The space distribution of specific chemical species was obtained by taking SNOM images at IR wavelengths (lambda) corresponding to stretch absorption bands of common biochemical bonds, such as the amide bond. In our SNOM implementation, this chemical sensitivity is combined with a lateral resolution of 0.1 micro m ( approximately lambda/70), well below the diffraction limit of standard infrared microscopy. The potential applications of this approach touch virtually every aspect of the life sciences and medical research, as well as problems in materials science, chemistry, physics, and environmental research.
Antonio Cricenti; Renato Generosi; Marco Luce; Paolo Perfetti; Giorgio Margaritondo; David Talley; Jas S Sanghera; Ishwar D Aggarwal; Norman H Tolk; Agostina Congiu-Castellano; Mark A Rizzo; David W Piston
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  85     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-25     Completed Date:  2004-06-08     Revised Date:  2010-09-21    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2705-10     Citation Subset:  IM    
Istituto di Stuttura della Materia, Rome, Italy.
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MeSH Terms
Bacteria / cytology*,  metabolism*
Biofilms / growth & development
Cell Line
Equipment Failure Analysis
Islets of Langerhans / cytology*,  metabolism*
Microscopy, Confocal / instrumentation*,  methods*
Spectrophotometry, Infrared / instrumentation*,  methods*

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