| Chemically defined medium for Legionella pneumophila growth. | |
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MedLine Citation:
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PMID: 7462408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A chemically defined medium containing 18 amino acids, inorganic salts, rhamnose, choline, and ferric pyrophosphate has been developed. The final concentrations of salts and amino acids were modeled after yeast extract. This medium supported the growth of four serogroups of Legionella pneumophila. Growth in shake cultures at 37 degrees C produced a lag time of approximately 5 h and a generation time of 4 h with a maximum growth yield of 10 9 colony-forming units per ml. A soluble brown pigment was observed in the stationary phase of growth. The optimal pH was 6.3. Rhamnose and choline were stimulatory; arginine, serine, threonine, cysteine, valine, and methionine were essential. Supplemental iron was not required to attain maximum growth, but iron deprivation caused an extended lag phase. |
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Authors:
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J D Ristroph; K W Hedlund; S Gowda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 13 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1981 Jan |
Date Detail:
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Created Date: 1981-04-13 Completed Date: 1981-04-13 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 115-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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pharmacology Choline / pharmacology Culture Media Ferric Compounds / pharmacology Hydrogen-Ion Concentration Ketoglutaric Acids / pharmacology Legionella / growth & development* Pyruvates / pharmacology Rhamnose / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Culture Media; 0/Ferric Compounds; 0/Ketoglutaric Acids; 0/Pyruvates; 10485-94-6/Rhamnose; 62-49-7/Choline |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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