| A Chemically Functionalized Magnetic Nanoplatform for Rapid and Specific Biomolecular Recognition and Separation. | |
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MedLine Citation:
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PMID: 23198853 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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We have developed a target-molecule-functionalized magnetic nanoparticle (MNP)-based method to facilitate the study of bio-molecular recognition and separation. The superparamagnetic property of MNPs allows the corresponding biomolecules to be rapidly separated from crude biofluids with a significant improvement in recovery yield and specificity. Various MNPs functionalized with tag molecules (chitin, heparin, and amylose) were synthesized for recombinant protein purification, and several probe-functionalized MNPs, such as nitrilotriacetic acid (NTA)@MNP and Pk@MNP, exhibited excellent extraction efficiency for proteins. In a cell recognition study, mannose-functionalized MNPs allowed specific purification of Escherichia coli with FimH adhesion on the surface. In an immunoprecipitation assay, the antibody-conjugated MNPs reduced the incubation time from 12 hours to 1 hour while maintaining a comparable efficiency. The functionalized MNPs were also used in a membrane proteomic study that utilized the interaction between streptavidin-functionalized MNPs and biotinylated cell membrane proteins. Overall, the functionalized MNPs were demonstrated to be promising probes for the specific separation of targets from proteins to cells and proteomics. |
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Authors:
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Po-Chiao Lin; Ching-Ching Yu; Huan-Ting Wu; Ying-Wei Lu; Chia-Li Han; An-Kai Su; Yu-Ju Chen; Chun-Cheng Lin |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-3 |
Journal Detail:
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Title: Biomacromolecules Volume: - ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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