Document Detail


A Chemically Functionalized Magnetic Nanoplatform for Rapid and Specific Biomolecular Recognition and Separation.
MedLine Citation:
PMID:  23198853     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We have developed a target-molecule-functionalized magnetic nanoparticle (MNP)-based method to facilitate the study of bio-molecular recognition and separation. The superparamagnetic property of MNPs allows the corresponding biomolecules to be rapidly separated from crude biofluids with a significant improvement in recovery yield and specificity. Various MNPs functionalized with tag molecules (chitin, heparin, and amylose) were synthesized for recombinant protein purification, and several probe-functionalized MNPs, such as nitrilotriacetic acid (NTA)@MNP and Pk@MNP, exhibited excellent extraction efficiency for proteins. In a cell recognition study, mannose-functionalized MNPs allowed specific purification of Escherichia coli with FimH adhesion on the surface. In an immunoprecipitation assay, the antibody-conjugated MNPs reduced the incubation time from 12 hours to 1 hour while maintaining a comparable efficiency. The functionalized MNPs were also used in a membrane proteomic study that utilized the interaction between streptavidin-functionalized MNPs and biotinylated cell membrane proteins. Overall, the functionalized MNPs were demonstrated to be promising probes for the specific separation of targets from proteins to cells and proteomics.
Authors:
Po-Chiao Lin; Ching-Ching Yu; Huan-Ting Wu; Ying-Wei Lu; Chia-Li Han; An-Kai Su; Yu-Ju Chen; Chun-Cheng Lin
Related Documents :
24156473 - Chemical tools to probe cellular o-glcnac signalling.
23027733 - Targeted proteomic dissection of toxoplasma cytoskeleton sub-compartments using morn1.
16138693 - Fluorimetric studies and noncovalent labeling of protein with the near-infrared dye hit...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-3
Journal Detail:
Title:  Biomacromolecules     Volume:  -     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Perinatal factors in non-disabled ELBW school children and later performance.
Next Document:  Ultrafast two-dimensional infrared spectroscopy resolves the conformational change of an Evans auxil...