| Chemical versus mechanical perturbations on the protonation state of arginine in complex lipid membranes: insights from microscopic pKa calculations. | |
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MedLine Citation:
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PMID: 20816065 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Charged amino acids such as Arginine play important roles in many membrane-mediated biological processes such as voltage gating of ion channels and membrane translocation of cell penetration peptides. It is well established that local membrane deformation and formation of water defects are crucial to the stabilization of charged species in contact with the membrane, which suggests that mechanical properties of the membrane are relevant although a clear connection has not been established. As a quantitative measure, we study how changes in the composition and therefore mechanical properties of a lipid bilayer influence the pK(a) of Arg in the membrane center using free energy simulations. Compared to previous studies in a single-component lipid bilayer containing saturated lipids or lipids with a modest degree of unsaturation, substantially larger pK(a) shifts are observed in the presence of highly unsaturated lipid tails and cholesterol. Moreover, the underlying molecular mechanisms for the pK(a) perturbation are distinct in different systems, with the unsaturated lipid tails mainly destabilizing the charged state of Arg and the cholesterol stabilizing the neutral state of Arg. The observed behaviors in both cases are at odds with predictions based on mechanical considerations at a mesoscopic level--highlighting that, while mechanical considerations are useful for stimulating hypothesis, their applicability to dissecting phenomena at the molecular-length scale is rather limited. |
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Authors:
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Jejoong Yoo; Qiang Cui |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Biophysical journal Volume: 99 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2010-12-17 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 1529-38 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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Graduate Program in Biophysics, University of Wisconsin-Madison, Madison, Wisconsin, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arginine
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chemistry*,
metabolism* Cell Membrane / chemistry, metabolism* Cholesterol / chemistry, metabolism Glycerophospholipids / chemistry, metabolism Lipid Bilayers / chemistry, metabolism* Mechanical Processes* Models, Molecular Molecular Conformation Physicochemical Processes* Protons* Thermodynamics |
| Grant Support | |
ID/Acronym/Agency:
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R01-GM071428/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glycerophospholipids; 0/Lipid Bilayers; 0/Protons; 57-88-5/Cholesterol; 74-79-3/Arginine |
| Comments/Corrections | |
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