Document Detail

Chemical treatment of carpets to reduce allergen: a detailed study of the effects of tannic acid on indoor allergens.
MedLine Citation:
PMID:  8027494     Owner:  NLM     Status:  MEDLINE    
Tannic acid (TA), a protein-denaturing agent, has been reported to reduce allergen levels in house dust and is marketed for that purpose as 1% and 3% solutions. We investigated the effects of TA on dust allergens by using monoclonal antibody-based ELISAs for mite (Der p I, Der f I, and group II) and cat (Fel d I) allergens. Initial studies confirmed that TA reduced allergen levels in carpet dust. However, when dust samples from treated carpets are extracted in saline solution, residual TA redissolves and may interfere with the assessment of allergens. In the laboratory, concentrations of TA as low as 0.1% inhibited the assays, but this effect may be prevented by addition of 5% bovine serum albumin (BSA). After treatment of dust samples in the laboratory with 3% TA, the apparent reductions in Der p I and Der f I levels were 89% and 96%, respectively, but when the samples were extracted in 5% BSA the reductions were 74% and 92%. Similar effects were seen with dust samples from carpets treated with TA. In an extreme case in which a carpet had been repeatedly treated with TA, the apparent concentration of Der p I was < 0.05 microgram/gm without BSA and 2.1 and 8.4 microgram/gm when extracted in the presence of 1% and 5% BSA, respectively. Our testing of the ability of TA to denature Fel d I demonstrated an 80% reduction in allergen, but only in samples with an initial concentration of less than 200 micrograms Fel d I/gm dust.(ABSTRACT TRUNCATED AT 250 WORDS)
J A Woodfolk; M L Hayden; J D Miller; G Rose; M D Chapman; T A Platts-Mills
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of allergy and clinical immunology     Volume:  94     ISSN:  0091-6749     ISO Abbreviation:  J. Allergy Clin. Immunol.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-10     Completed Date:  1994-08-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1275002     Medline TA:  J Allergy Clin Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  19-26     Citation Subset:  AIM; IM    
Department of Medicine, University of Virginia Health Sciences Center, Charlottesville 22908.
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MeSH Terms
Air Pollution, Indoor / analysis,  prevention & control*
Allergens / analysis,  drug effects*
Antigens, Dermatophagoides
Cockroaches / immunology
Dose-Response Relationship, Drug
Drug Interactions
Dust / analysis
Enzyme-Linked Immunosorbent Assay
Floors and Floorcoverings*
Glycoproteins / analysis,  drug effects
Hydrolyzable Tannins / analysis,  pharmacology*
Mites / immunology
Grant Support
Reg. No./Substance:
0/Allergens; 0/Antigens, Dermatophagoides; 0/Dust; 0/Fel d 1 protein, Felis domesticus; 0/Glycoproteins; 0/Hydrolyzable Tannins; 0/Solutions

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