| Chemical synthesis of nucleoside-gamma-[32P]triphosphates of high specific activity. | |
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MedLine Citation:
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PMID: 7375446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple chemical procedure for the preparation of four common ribonucleoside 5-gamma-[32P]triphosphates of high specific activity (up to 10 Ci/mmole) based on the condensation of orthophosphoric acid with the corresponding nucleoside 5-diphosphate in the presence of ethyl chloroformate as well as the methods of purification and identification of the products are described. |
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Authors:
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A Janecka; H Panusz; J Pankowski; W Koziołkiewicz |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Preparative biochemistry Volume: 10 ISSN: 0032-7484 ISO Abbreviation: Prep. Biochem. Publication Date: 1980 |
Date Detail:
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Created Date: 1980-07-12 Completed Date: 1980-07-12 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 1276634 Medline TA: Prep Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 27-35 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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analysis,
chemical synthesis* Cytidine Triphosphate / analysis, chemical synthesis* Cytosine Nucleotides / chemical synthesis* Guanosine Triphosphate / analysis, chemical synthesis* Isotope Labeling Methods Phosphorus Radioisotopes Uracil Nucleotides / chemical synthesis* Uridine Triphosphate / analysis, chemical synthesis* |
| Chemical | |
Reg. No./Substance:
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0/Cytosine Nucleotides; 0/Phosphorus Radioisotopes; 0/Uracil Nucleotides; 56-65-5/Adenosine Triphosphate; 63-39-8/Uridine Triphosphate; 65-47-4/Cytidine Triphosphate; 86-01-1/Guanosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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