Document Detail

Chemical specificity of effector cell/tumor cell bridging by a Viscum album rhamnogalacturonan enhancing cytotoxicity of human NK cells.
MedLine Citation:
PMID:  2407685     Owner:  NLM     Status:  MEDLINE    
The component in Viscum album extract Iscador-M enhancing the NK cytotoxicity of human CD56+CD3- NK cells (87-95% enrichment) in cocultures with K562 tumor cells and increasing the formation of NK cell/tumor cell conjugates was identified as a rhamnogalacturonan. Both activities were abolished by treatment of V. album extract with poly-alpha-D-galacturonidase and alpha-rhamnosidase and both activities were inhibited in the presence of galacturonic acid and acetylated rhamnose (6-deoxymannose). Inhibition was also observed in the presence of structurally related derivatives such as acetylated mannose or acetylated mannonic acid gamma-lactone, the latter exhibiting a 5-10-fold higher inhibitory potential. The rapid formation of NK cell/tumor cell conjugates in the presence of V. album extract was based on the bridging of NK cells with tumor cells by rhamnogalacturonan. Using a specifically adapted agglutination assay, the saccharide residues of the rhamnogalacturonan interacting with NK cells and tumor cells could be identified by the formation of homologous cell conjugates induced by acetylated rhamnose or acetylated mannose conjugated to dextran and by polygalacturonic acid: terminal acetylated rhamnose or acetylated mannose bound only to NK cells in a dose-dependent manner but not to K562 tumor cells, and terminal galacturonic acid only to K562 tumor cells but not to NK cells. This type of bridging represents a novel mechanism of enhancement of NK cytotoxicity.
E A Mueller; F A Anderer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunopharmacology     Volume:  19     ISSN:  0162-3109     ISO Abbreviation:  Immunopharmacology     Publication Date:    1990 Jan-Feb
Date Detail:
Created Date:  1990-04-10     Completed Date:  1990-04-10     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7902474     Medline TA:  Immunopharmacology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  69-77     Citation Subset:  IM    
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tübingen, F.R.G.
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MeSH Terms
Agglutination Tests
Antineoplastic Agents, Phytogenic / pharmacology*
Cell Survival / drug effects*
Fluorescent Antibody Technique
Killer Cells, Natural / drug effects*,  immunology
L-Lactate Dehydrogenase / analysis,  metabolism
Monocytes / drug effects
Plant Extracts / pharmacology*
Plant Proteins*
Plants, Medicinal*
Tumor Cells, Cultured / drug effects,  immunology
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Plant Extracts; 0/Plant Proteins; 0/viscum album peptide; EC Dehydrogenase

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