Document Detail

Chemical and serological relationships between the heteropolysaccharides of Mycobacterium tuberculosis and Mycobacterium kansasii.
MedLine Citation:
PMID:  4966549     Owner:  NLM     Status:  MEDLINE    
The identity of a heteropolysaccharide from cell walls of Mycobacterium tuberculosis H37Ra with Seibert's tuberculopolysaccharide I was demonstrated by thin-layer chromatography, chemical analysis, and antigenic tests. The polysaccharide of M. kansasii was shown to be identical with that of M. tuberculosis. Defatted cells were disintegrated by ultrasonic treatment in the presence of glass beads; cell walls were obtained by differential ultracentrifugation. Ethyl alcohol-precipitated carbohydrate extracts were analyzed for protein and nucleic acid; these impurities were removed. Tuberculopolysaccharide I from the mycobacterial culture filtrate is probably derived from a lipopolysaccharide of the cell wall, which is partially removed by chloroform in the intact state. Alkaline extraction releases additional polysaccharide, in varying degrees of association with cell wall murein.
S E Birnbaum; L F Affronti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  95     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1968 Feb 
Date Detail:
Created Date:  1968-05-03     Completed Date:  1968-05-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  559-64     Citation Subset:  IM    
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MeSH Terms
Arabinose / analysis
Cell Wall / analysis
Chromatography, Thin Layer
Hexosamines / analysis
Hexoses / analysis
Mycobacterium / analysis*
Mycobacterium tuberculosis / analysis*
Polysaccharides, Bacterial / analysis*
Precipitin Tests
Reg. No./Substance:
0/Hexosamines; 0/Hexoses; 0/Polysaccharides, Bacterial; 147-81-9/Arabinose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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