Document Detail


Chemical and serological relationships between the heteropolysaccharides of Mycobacterium tuberculosis and Mycobacterium kansasii.
MedLine Citation:
PMID:  4966549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The identity of a heteropolysaccharide from cell walls of Mycobacterium tuberculosis H37Ra with Seibert's tuberculopolysaccharide I was demonstrated by thin-layer chromatography, chemical analysis, and antigenic tests. The polysaccharide of M. kansasii was shown to be identical with that of M. tuberculosis. Defatted cells were disintegrated by ultrasonic treatment in the presence of glass beads; cell walls were obtained by differential ultracentrifugation. Ethyl alcohol-precipitated carbohydrate extracts were analyzed for protein and nucleic acid; these impurities were removed. Tuberculopolysaccharide I from the mycobacterial culture filtrate is probably derived from a lipopolysaccharide of the cell wall, which is partially removed by chloroform in the intact state. Alkaline extraction releases additional polysaccharide, in varying degrees of association with cell wall murein.
Authors:
S E Birnbaum; L F Affronti
Related Documents :
6393889 - Cytochemical and ultrastructural studies of candida albicans. iii. evidence for modific...
6347209 - Microscopic studies of candida albicans and torulopsis glabrata after in vitro treatmen...
11500569 - Transpiration rate. an important factor controlling the sucrose content of the guard ce...
6045299 - Inhibition of rna synthesis and auxin-induced cell wall extensibility and growth by act...
9685659 - Dielectric behavior of budding yeast in cell separation.
18001349 - The sun41 and sun42 genes are essential for cell separation in candida albicans.
199259 - Synthesis and secretion of albumin by a synchronized rat hepatoma cell line.
20709899 - Identification, source, and metabolism of n-ethylglutamate in escherichia coli.
6371029 - Regulation of mitotic activity and the cell cycle in primary chick muscle cells by neur...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  95     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1968 Feb 
Date Detail:
Created Date:  1968-05-03     Completed Date:  1968-05-03     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  559-64     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Arabinose / analysis
Cell Wall / analysis
Chromatography, Thin Layer
Hexosamines / analysis
Hexoses / analysis
Immunodiffusion
Mycobacterium / analysis*
Mycobacterium tuberculosis / analysis*
Polysaccharides, Bacterial / analysis*
Precipitin Tests
Ultracentrifugation
Ultrasonics
Chemical
Reg. No./Substance:
0/Hexosamines; 0/Hexoses; 0/Polysaccharides, Bacterial; 147-81-9/Arabinose
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Correlation between mutation type and the production of cross-reacting material in mutants of the A ...
Next Document:  Dependence of diaminopurine utilization on the mutational site of purine auxotrophy in Bacillus subt...