Document Detail


Chemical sensing in mammalian host-bacterial commensal associations.
MedLine Citation:
PMID:  20457895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mammalian gastrointestinal (GI) tract is colonized by a complex consortium of bacterial species. Bacteria engage in chemical signaling to coordinate population-wide behavior. However, it is unclear if chemical sensing plays a role in establishing mammalian host-bacterial commensal relationships. Enterohemorrhagic Escherichia coli (EHEC) is a deadly human pathogen but is a member of the GI flora in cattle, its main reservoir. EHEC harbors SdiA, a regulator that senses acyl-homoserine lactones (AHLs) produced by other bacteria. Here, we show that SdiA is necessary for EHEC colonization of cattle and that AHLs are prominent within the bovine rumen but absent in other areas of the GI tract. We also assessed the rumen metagenome of heifers, and we show that it is dominated by Clostridia and/or Bacilli but also harbors Bacteroidetes. Of note, some members of the Bacteroidetes phyla have been previously reported to produce AHLs. SdiA-AHL chemical signaling aids EHEC in gauging these GI environments, and promotes adaptation to a commensal lifestyle. We show that chemical sensing in the mammalian GI tract determines the niche specificity for colonization by a commensal bacterium of its natural animal reservoir. Chemical sensing may be a general mechanism used by commensal bacteria to sense and adapt to their mammalian hosts. Additionally, because EHEC is largely prevalent in cattle herds, interference with SdiA-mediated cattle colonization is an exciting alternative to diminish contamination of meat products and cross-contamination of produce crops because of cattle shedding of this human pathogen.
Authors:
David T Hughes; Darya A Terekhova; Linda Liou; Carolyn J Hovde; Jason W Sahl; Arati V Patankar; Juan E Gonzalez; Thomas S Edrington; David A Rasko; Vanessa Sperandio
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2010-05-10
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  107     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-26     Completed Date:  2010-06-29     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9831-6     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl-Butyrolactones / metabolism*
Animals
Cattle / microbiology*
Enterohemorrhagic Escherichia coli / genetics,  physiology*
Escherichia coli Proteins / genetics,  metabolism*
Gene Expression Regulation, Bacterial
Host-Pathogen Interactions*
Humans
Mutation
Phosphoproteins / genetics,  metabolism
Rumen / microbiology*
Signal Transduction
Trans-Activators / genetics,  metabolism*
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
5-T32-AI007520-07/AI/NIAID NIH HHS; AI053067/AI/NIAID NIH HHS; AI077613/AI/NIAID NIH HHS; P20-RR16454/RR/NCRR NIH HHS; R01 AI077613/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Acyl-Butyrolactones; 0/Escherichia coli Proteins; 0/LEE protein, E coli; 0/Phosphoproteins; 0/Trans-Activators; 0/sdiA protein, E coli
Comments/Corrections
Erratum In:
Proc Natl Acad Sci U S A. 2010 Jul 13;107(28):12734
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10765

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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