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Chemical profiling of Edelweiss (Leontopodium alpinum Cass.) extracts by Micellar Electrokinetic Capillary Chromatography.
MedLine Citation:
PMID:  23041520     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This manuscript reports on the first CE-method suitable for analyzing twelve pharmacologically relevant compounds (flavonoids, caffeic acids and leontopodic acid) in the aerial parts of Leontopodium alpinum. By using a 60mM borax buffer containing 25mM SDS and 35% acetonitrile the analytes could be well resolved in 25min and accurately determined in different Edelweiss specimens. Method validation assured compliance of the assay to ICH standards (correlation coefficients≥0.999, LOD≤7.2μg/ml, LOQ≤23.0μg/ml, recovery rates from 96.6 to 101.1%, intermediate precision≤7.01%, and repeatability≤5.52%). The quantitative results revealed a strong dependence of the content of phenolics on the growth status of the plants. In early stages no flavonoids were found, while during blooming all metabolites reached higher levels; commercially cultivated plant material showed the overall highest values (flavonoids: 0.25 - 0.34%, caffeic acids: 4.99 - 6.21%, leontopodic acid: 4.21 - 5.05%).
Authors:
Markus Ganzera; Viktoria Greifeneder; Stefan Schwaiger; Hermann Stuppner
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-4
Journal Detail:
Title:  Fitoterapia     Volume:  -     ISSN:  1873-6971     ISO Abbreviation:  Fitoterapia     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  16930290R     Medline TA:  Fitoterapia     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Institute of Pharmacy, Pharmacognosy, University of Innsbruck, CCB, Innrain 80-82, 6020 Innsbruck, Austria. Electronic address: Markus.Ganzera@uibk.ac.at.
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