| Chemical profiling of Edelweiss (Leontopodium alpinum Cass.) extracts by Micellar Electrokinetic Capillary Chromatography. | |
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MedLine Citation:
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PMID: 23041520 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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This manuscript reports on the first CE-method suitable for analyzing twelve pharmacologically relevant compounds (flavonoids, caffeic acids and leontopodic acid) in the aerial parts of Leontopodium alpinum. By using a 60mM borax buffer containing 25mM SDS and 35% acetonitrile the analytes could be well resolved in 25min and accurately determined in different Edelweiss specimens. Method validation assured compliance of the assay to ICH standards (correlation coefficients≥0.999, LOD≤7.2μg/ml, LOQ≤23.0μg/ml, recovery rates from 96.6 to 101.1%, intermediate precision≤7.01%, and repeatability≤5.52%). The quantitative results revealed a strong dependence of the content of phenolics on the growth status of the plants. In early stages no flavonoids were found, while during blooming all metabolites reached higher levels; commercially cultivated plant material showed the overall highest values (flavonoids: 0.25 - 0.34%, caffeic acids: 4.99 - 6.21%, leontopodic acid: 4.21 - 5.05%). |
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Authors:
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Markus Ganzera; Viktoria Greifeneder; Stefan Schwaiger; Hermann Stuppner |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-10-4 |
Journal Detail:
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Title: Fitoterapia Volume: - ISSN: 1873-6971 ISO Abbreviation: Fitoterapia Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-8 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 16930290R Medline TA: Fitoterapia Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier B.V. |
Affiliation:
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Institute of Pharmacy, Pharmacognosy, University of Innsbruck, CCB, Innrain 80-82, 6020 Innsbruck, Austria. Electronic address: Markus.Ganzera@uibk.ac.at. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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