Document Detail


Chemical, physical, and biological characterization of a dimeric form of biosynthetic human growth hormone.
MedLine Citation:
PMID:  3440058     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A dimer of biosynthetic human growth hormone (HGH) has been isolated and characterized. This entity, which is the predominant dimeric species in biosynthetic HGH, is chemically identical to monomeric HGH and exists in a noncovalent dimeric form which is dissociated to monomeric HGH on polyacrylamide electrophoresis gels or in aqueous solutions containing 30% acetonitrile. This substance, found in all production lots of pituitary HGH, biosynthetic HGH, and biosynthetic methionyl HGH examined, is much less biopotent than monomeric HGH and can be distinguished from monomeric HGH by a monoclonal antibody. These data demonstrate that polyacrylamide gel electrophoresis is not a valid method for measuring this dimer and that size-exclusion chromatography under aqueous conditions is required.
Authors:
G W Becker; R R Bowsher; W C Mackellar; M L Poor; P M Tackitt; R M Riggin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  9     ISSN:  0885-4513     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-04-18     Completed Date:  1988-04-18     Revised Date:  2007-03-21    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  478-87     Citation Subset:  IM    
Affiliation:
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Animals
Biological Assay
Body Weight / drug effects
Bone Development / drug effects
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Female
Growth Hormone* / biosynthesis,  pharmacology
Humans
Hypophysectomy
Immunoassay
Macromolecular Substances
Molecular Weight
Rats
Rats, Inbred Strains
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Macromolecular Substances; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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