Document Detail

Chemical and immunochemical identification of propanoyllysine derived from oxidized n-3 polyunsaturated fatty acid.
MedLine Citation:
PMID:  19272447     Owner:  NLM     Status:  MEDLINE    
It is known that n-3 polyunsaturated fatty acids (PUFAs), such as docosahexaenoic acid and eicosapentaenoic acid, are rapidly oxidized in vitro. Nvarepsilon-(propanoyl)lysine (propionyllysine, or PRL) is formed from the reaction of the oxidized products of n-3 PUFAs and lysine. To evaluate the oxidized n-3 PUFA-derived protein modifications in vivo, we have developed detection methods using a novel monoclonal antibody against PRL as well as liquid chromatography-mass spectrometry (LC/MS/MS). The antibody obtained specifically recognized PRL. A strong positive staining in atherosclerotic lesions of hypercholesterolemic rabbits was observed. We have also simultaneously identified and quantified both urinary PRL and urinary Nvarepsilon-(hexanoyl)lysine, using LC/MS/MS using isotope dilution methods. The level of urinary PRL (21.6+/-10.6 micromol/mol of creatinine) significantly correlated with the other oxidative stress markers, 8-oxo-deoxyguanosine, dityrosine, and isoprostanes. The increase in the excretion of amide adducts into the urine of diabetic patients was also confirmed compared to healthy subjects. These results suggest that PRL may be good marker for n-3 PUFA-derived oxidative stress in vivo.
Shinsuke Hisaka; Yoji Kato; Noritoshi Kitamoto; Akihiro Yoshida; Yoshiko Kubushiro; Michitaka Naito; Toshihiko Osawa
Related Documents :
2141757 - Dose-response effects of fish-oil supplementation in healthy volunteers.
11724457 - The role of polyunsaturated fatty acid supplementation in intestinal inflammation and n...
11451717 - Effects of a high-dose concentrate of n-3 fatty acids or corn oil introduced early afte...
8565757 - Influence of administration of long-chain polyunsaturated fatty acids on process of his...
12552527 - Indanoyl amino acid conjugates: tunable elicitors of plant secondary metabolism.
17951687 - A mitosome purification protocol based on percoll density gradients and its use in vali...
Publication Detail:
Type:  Journal Article     Date:  2009-03-09
Journal Detail:
Title:  Free radical biology & medicine     Volume:  46     ISSN:  1873-4596     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-05-04     Completed Date:  2009-12-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1463-71     Citation Subset:  IM    
Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal / immunology
Aorta / immunology,  metabolism*,  pathology
Biological Markers / urine
Coronary Artery Disease / immunology,  metabolism*,  pathology
Deoxyguanosine / analogs & derivatives,  urine
Diabetes Insipidus / physiopathology,  urine
Dinoprost / analogs & derivatives,  urine
Fatty Acids, Unsaturated / chemistry,  metabolism
Hypercholesterolemia / immunology,  metabolism*,  pathology
Lysine / analogs & derivatives,  chemistry,  immunology,  metabolism
Mass Spectrometry
Oxidative Stress
Propionates / chemistry*,  immunology*,  metabolism
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Biological Markers; 0/Fatty Acids, Unsaturated; 0/Propionates; 0/propanoyllysine; 27415-26-5/8-epi-prostaglandin F2alpha; 551-11-1/Dinoprost; 56-87-1/Lysine; 88847-89-6/8-oxo-7-hydrodeoxyguanosine; 961-07-9/Deoxyguanosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Huperzine A protects isolated rat brain mitochondria against beta-amyloid peptide.
Next Document:  Mammalian target of rapamycin complex 1-mediated phosphorylation of eukaryotic initiation factor 4E-...