| Chemical hypoxia increases cytosolic Ca2+ and oxygen free radical formation. | |
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MedLine Citation:
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PMID: 7664318 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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'Chemical hypoxia' was produced in isolated rat hepatocytes. The cells were immobilized in agarose gel threads and perfused with Krebs-Henseleit bicarbonate buffer equilibrated with 95% O2 + 5% CO2 or 95% air + 5% CO2. During 'chemical hypoxia', 2 mM KCN + 0.5 mM iodoacetate (CN-IAA) were added to the perfusate for 30 min. Cytosolic ionized Ca2+ (Cai2+) was measured with aequorin, the formation of oxygen free radicals by lucigenin-enhanced chemiluminescence and cell injury by the rate of LDH released by the cells in the effluent perfusate. As soon as the cells were exposed to CN-IAA in the presence of 95% O2 + 5% CO2, Cai2+ increased rapidly to reach 1.5 microM within 10 min, and oxygen free radical formation increased 5-fold. The increase in LDH release was temporally delayed and occurred only during the recovery phase. The results were not significantly different when the cells were perfused with KHB equilibrated with 95% air + 5% CO2, except that oxygen free radical formation increased 13-fold. These results suggest that both a rise in Cai2+ and a formation of reactive oxygen species could be responsible for the cell injury and the cell death induced by CN-IAA poisoning. |
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Authors:
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A B Borle; M Barsic |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell calcium Volume: 17 ISSN: 0143-4160 ISO Abbreviation: Cell Calcium Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-10-10 Completed Date: 1995-10-10 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8006226 Medline TA: Cell Calcium Country: SCOTLAND |
Other Details:
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Languages: eng Pagination: 307-15 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pennsylvania, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport Calcium / metabolism* Cell Hypoxia* Cells, Cultured Chemiluminescent Measurements Cytosol / metabolism* Free Radicals Iodoacetates / pharmacology Iodoacetic Acid L-Lactate Dehydrogenase / analysis Liver / cytology, drug effects*, metabolism Male Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism* Sodium Cyanide / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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R01 DK 46935-01/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Free Radicals; 0/Iodoacetates; 0/Reactive Oxygen Species; 143-33-9/Sodium Cyanide; 64-69-7/Iodoacetic Acid; 7440-70-2/Calcium; EC 1.1.1.27/L-Lactate Dehydrogenase |
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