Document Detail


Chemical and histochemical studies of normal and diseased human gastrointestinal tract. V. A differential diagnostic method for the histochemical classification of glycoproteins.
MedLine Citation:
PMID:  2415487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A differential diagnostic scheme is described for the division of colonic epithelial glycoproteins into eleven histochemically distinct classes. The scheme depends upon the use of seven histochemical techniques which, collectively, permit the differential staining of O-sulphate ester, sialic acid and its side chain O-acyl variants and vicinal diols located on carbohydrate residues other than sialic acids. Elements of the scheme also provide a general approach to the classification of epithelial glycoproteins in anatomic sites other than the colon. Application of the scheme permitted the classification of the epithelial glycoproteins in the mucosa 0.5-5.0 cm from human colonic tumours and provided direct confirmation of previous observations that changes from normal in the relative proportions of either side chain O-acylated sialic acids or sialic acids and O-sulphate esters can occur independently of one another.
Authors:
P E Reid; D A Owen; C W Ramey; W L Dunn; E A Jones; D A Lazosky; E Allen; C M Park; M G Clay
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Histochemical journal     Volume:  17     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1985-12-30     Completed Date:  1985-12-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  891-903     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Colonic Neoplasms / diagnosis
Diagnosis, Differential
Digestive System / analysis*
Glycoproteins / analysis*,  classification
Histocytochemistry
Humans
Intestinal Mucosa / enzymology
Staining and Labeling
Chemical
Reg. No./Substance:
0/Glycoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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