Document Detail

Chemical enhancement of SA7 virus transformation of hamster embryo cells: evaluation by interlaboratory testing of diverse chemicals.
MedLine Citation:
PMID:  3732194     Owner:  NLM     Status:  MEDLINE    
Twelve chemicals from diverse structural classes were tested under code for their capacity to enhance the transformation of Syrian hamster embryo cells by simian adenovirus SA7 in two independent laboratories. Pretreatment of hamster cells with eight of those chemicals (reserpine, dichlorvos, methapyrilene hydrochloride, benzidine dihydrochloride, diphenylhydantoin, cinnamyl anthranilate, 11-aminoundecanoic acid, and 4,4'-oxydianiline) produced repeatable enhancement of SA7 transformation at two or more consecutive dose levels, which constitutes clear evidence of enhancing activity in this assay. Both toxic and nontoxic doses of each of these chemicals caused enhancement of virus transformation. Two chemicals (2,6-dichloro-p-phenylenediamine and cinnamaldehyde) produced some evidence of enhancing activity (repeatable transformation enhancement at one dose). Dose ranges for cytotoxicity and enhancement of SA7 transformation were similar in both laboratories for all chemicals producing activity. The final two chemicals, chloramphenicol sodium succinate and ethylene thiourea, failed to reproducibly demonstrate either significant cytotoxicity or enhancement of SA7 transformation at concentrations up to 10-20 mM. The test results for these 12 chemicals were combined with the test results for 9 known carcinogens and noncarcinogens in order to evaluate relationships between activity, dose response, and lowest effective enhancing concentration for these compounds, as well as to correlate them with rodent carcinogenesis classifications. The Syrian hamster embryo cell-SA7 system demonstrated reproducible test responses in both intra- and interlaboratory studies and detected 13 out of 15 known rodent carcinogens.
G G Hatch; T M Anderson; R A Lubet; R E Kouri; D L Putman; J W Cameron; R W Nims; B Most; J W Spalding; R W Tennant
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Environmental mutagenesis     Volume:  8     ISSN:  0192-2521     ISO Abbreviation:  Environ Mutagen     Publication Date:  1986  
Date Detail:
Created Date:  1986-09-23     Completed Date:  1986-09-23     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7909737     Medline TA:  Environ Mutagen     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  515-31     Citation Subset:  IM    
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MeSH Terms
Adenoviridae / pathogenicity*
Adenoviruses, Simian / pathogenicity*
Cell Transformation, Neoplastic / drug effects*
Cell Transformation, Viral / drug effects*
Cells, Cultured
Dose-Response Relationship, Drug
Embryo, Mammalian
Grant Support
Reg. No./Substance:

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