Document Detail

Chemical composition and cytotoxic, mutagenic and genotoxic activities of the essential oil from Pipergaudichaudianum Kunth leaves.
MedLine Citation:
PMID:  19563855     Owner:  NLM     Status:  MEDLINE    
We have investigated the chemical composition of Piper gaudichaudianum essential oil, as well as its cytotoxic, mutagenic and genotoxic effects in V79 cells. The chemical analyses showed that the major compounds are (E)-nerolidol (22.4%), alpha-humulene (16.5%), (E)-caryophyllene (8.9%) and bicyclogermacrene (7.4%). Dose-dependent cytotoxic effects were observed in V79 cells treated with essential oil by using clonal survival, 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide reduction assay (MTT) and trypan blue exclusion assay (TB), and a significant decrease in survival was observed at concentrations of 0.5 microg/mL and higher. The P. gaudichaudianum essential oil treatment caused DNA strand breaks in V79 cells at concentrations up to 2 microg/mL, as detected by the alkaline comet assay, but did not induce double-strand breaks, as verified by neutral comet assay. It induced a significant increase in the frequency of micronucleated cells at 4, 6 and 10 microg/mL. Moreover, P. gaudichaudianum essential oil significantly increased lipid peroxidation at doses of 0.5 microg/mL and higher, suggesting that the observed oxidant potential can be responsible, at least in part, for its cytotoxic and genotoxic effects.
V F Péres; D J Moura; A R M Sperotto; F C Damasceno; E B Caramão; C A Zini; J Saffi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-27
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  47     ISSN:  1873-6351     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2389-95     Citation Subset:  IM    
Laboratório de Genética Toxicológica, Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil, Canoas, RS, Brazil.
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MeSH Terms
Cell Line
Cell Survival / drug effects
Comet Assay
DNA Damage
Dose-Response Relationship, Drug
Fibroblasts / drug effects*,  metabolism,  pathology
Gas Chromatography-Mass Spectrometry
Lipid Peroxidation / drug effects
Lung / drug effects*,  pathology
Malondialdehyde / metabolism
Micronuclei, Chromosome-Defective / chemically induced
Micronucleus Tests
Mutagens / analysis,  toxicity*
Oils, Volatile / analysis,  toxicity*
Piperaceae / chemistry*
Plant Leaves / chemistry
Plant Oils / analysis,  toxicity*
Thiobarbituric Acid Reactive Substances / metabolism
Reg. No./Substance:
0/Mutagens; 0/Oils, Volatile; 0/Plant Oils; 0/Thiobarbituric Acid Reactive Substances; 542-78-9/Malondialdehyde

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