| Chemical characterization of a rabbit leukocytic pyrogen. | |
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MedLine Citation:
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PMID: 7140775 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Crude pyrogen was obtained by stimulating 1.4 x 10(12) peritoneal-exudate leukocytes from rabbits in vitro with Escherichia coli endotoxin. A pyrogen molecule was isolated by ammonium-sulfate precipitation, polyacrylamide gel electrophoresis at pH 5.5 and 8.9, and gel filtration on Bio-Gel P-100. These procedures yielded a pyrogenic fraction which was electrophoretically and immunologically homogeneous. The pyrogenicity was 50 ng/kg of rabbit. This leukocyte pyrogen consisted of a protein with a molecular weight of approximately 14 000. Amino-acid analysis showed high amounts of glutamic acid and glycine; methionine and tryptophan were present in small quantities. Arginine was found to be the N-terminal amino acid. The molecule contained about 0.8% neutral sugar and 0.7% lipids. Its approximate isoelectric point in citrate/phosphate buffer was 4.2. The activity was lost by lyophilization and storage at - 18 degrees C for six months. The data presented here are the first detailed characterization of a rabbit leukocyte pyrogen. The properties in common to leukocytic pyrogen and interleukin 1 are discussed. |
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Authors:
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F Pacák; R Siegert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 127 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1982 Oct |
Date Detail:
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Created Date: 1983-01-19 Completed Date: 1983-01-19 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 375-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Chemical Phenomena Chemistry Interleukin-1* Molecular Weight Proteins / isolation & purification* Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Interleukin-1; 0/Proteins; 0/leukocyte endogenous mediator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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