| Chemical characterization of p17gag from human immunodeficiency virus as an N-terminally myristoylated protein. | |
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MedLine Citation:
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PMID: 2737204 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The N-terminal p17gag protein of the human immunodeficiency virus has been shown to incorporate radioactivity following labelling of infected cell lines with [3H]myristic acid. We investigated p17gag to determine whether the incorporated radioactivity was the consequence of N-terminal myristoylation. The virus was purified by density gradient centrifugation after labelling chronically infected H9 cells with [3H]myristic acid. The p17gag was isolated by immunoprecipitation and subjected to partial acid hydrolysis. [3H]Myristoylglycine generated by the hydrolysis was derivatized to 4-(p-nitrobenzylidene)-2-tridecanoyloxazol-5-one and identified against a co-eluting, derivatized, unlabelled N-myristoylglycine standard by reverse-phase high-performance liquid chromatography. This study unequivocally demonstrates that p17gag is an N-myristoylated protein. |
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Authors:
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C Goddard; A Aquino; R I Glazer; R L Felsted |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 182 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1989 Jun |
Date Detail:
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Created Date: 1989-08-04 Completed Date: 1989-08-04 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 323-6 Citation Subset: IM; X |
Affiliation:
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Division of Cancer Treatment, National Cancer Institute, Bethesda, MD 20892. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Gene Products, gag* HIV / analysis*, isolation & purification HIV Antigens / analysis* Humans Isotope Labeling Myristic Acid Myristic Acids / analysis*, metabolism Peptide Chain Termination, Translational Precipitin Tests Retroviridae Proteins / analysis* Viral Proteins* gag Gene Products, Human Immunodeficiency Virus |
| Chemical | |
Reg. No./Substance:
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0/Gene Products, gag; 0/HIV Antigens; 0/Myristic Acids; 0/Retroviridae Proteins; 0/Viral Proteins; 0/gag Gene Products, Human Immunodeficiency Virus; 0/p17 protein, Human Immunodeficiency Virus Type 1; 544-63-8/Myristic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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