Document Detail

Chemical characterization of p17gag from human immunodeficiency virus as an N-terminally myristoylated protein.
MedLine Citation:
PMID:  2737204     Owner:  NLM     Status:  MEDLINE    
The N-terminal p17gag protein of the human immunodeficiency virus has been shown to incorporate radioactivity following labelling of infected cell lines with [3H]myristic acid. We investigated p17gag to determine whether the incorporated radioactivity was the consequence of N-terminal myristoylation. The virus was purified by density gradient centrifugation after labelling chronically infected H9 cells with [3H]myristic acid. The p17gag was isolated by immunoprecipitation and subjected to partial acid hydrolysis. [3H]Myristoylglycine generated by the hydrolysis was derivatized to 4-(p-nitrobenzylidene)-2-tridecanoyloxazol-5-one and identified against a co-eluting, derivatized, unlabelled N-myristoylglycine standard by reverse-phase high-performance liquid chromatography. This study unequivocally demonstrates that p17gag is an N-myristoylated protein.
C Goddard; A Aquino; R I Glazer; R L Felsted
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  182     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-08-04     Completed Date:  1989-08-04     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  323-6     Citation Subset:  IM; X    
Division of Cancer Treatment, National Cancer Institute, Bethesda, MD 20892.
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MeSH Terms
Cell Line
Centrifugation, Density Gradient
Electrophoresis, Polyacrylamide Gel
Gene Products, gag*
HIV / analysis*,  isolation & purification
HIV Antigens / analysis*
Isotope Labeling
Myristic Acid
Myristic Acids / analysis*,  metabolism
Peptide Chain Termination, Translational
Precipitin Tests
Retroviridae Proteins / analysis*
Viral Proteins*
gag Gene Products, Human Immunodeficiency Virus
Reg. No./Substance:
0/Gene Products, gag; 0/HIV Antigens; 0/Myristic Acids; 0/Retroviridae Proteins; 0/Viral Proteins; 0/gag Gene Products, Human Immunodeficiency Virus; 0/p17 protein, Human Immunodeficiency Virus Type 1; 544-63-8/Myristic Acid

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