Document Detail


Chemical characterization of the hen eggshell matrix: isolation of an alkali-resistant peptide.
MedLine Citation:
PMID:  7068793     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The eggshell matrix was obtained from hen eggshells using EDTA solutions. The water-soluble organic material was separated on a DEAE-cellulose column equilibrated with 8 M urea using Tris-hydrochloric acid buffer as eluent with a linear gradient of sodium chloride. Five main fractions were obtained which differ in amino acid composition and sugar contents. As is shown from the uronic acid content, the first two fractions eluted from the column are glycoproteins, while the other three contain proteins and glycosaminoglycans From the alkaline hydrolysate of the eggshell matrix, a peptide was isolated which is composed of aspartic acid, threonine, serine, glutamic acid, proline, glycine and alanine in a molar ratio of 2:1:3:7:1:3:1 with a minimum molecular weight of 2158 daltons. The calcium ion binding to this peptide was studied, at different pH values, with both free and blocked carboxyl groups, using murexide as an indicator of free Ca2+. The importance of this acidic peptide in the calcification process of the eggshell matrix is discussed.
Authors:
R Cortivo; I Castellani; M Martelli; G Michelotto; G Abatangelo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatography     Volume:  237     ISSN:  -     ISO Abbreviation:  J. Chromatogr.     Publication Date:  1982 Mar 
Date Detail:
Created Date:  1982-06-14     Completed Date:  1982-06-14     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0427043     Medline TA:  J Chromatogr     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  127-35     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis,  metabolism
Animals
Calcification, Physiologic
Calcium / metabolism*
Chickens
Chromatography, DEAE-Cellulose
Egg Shell / analysis*,  metabolism
Female
Hydrogen-Ion Concentration
Peptides / isolation & purification*,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Peptides; SY7Q814VUP/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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