Document Detail


Chemical characterization of enterobacterial common antigen isolated from Plesiomonas shigelloides ATCC 14029.
MedLine Citation:
PMID:  3816787     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Serologically characterized samples of enterobacterial common antigen (ECA) from Plesiomonas shigelloides, Salmonella montevideo and Shigella sonnei were investigated by chemical methods including methylation and NMR techniques. All showed the same sugar composition and contained a lipid moiety with palmitic acid as main fatty acid and with a phosphodiester group. Additional enzymatic studies, reported in the preceding paper, provided evidence that the lipid moiety is an L-glycerophosphatidyl residue attached via a phosphodiester linkage to C-1 of GlcNAc as the reducing end of the ECA sugar chain. ECA of P. shigelloides showed the best-resolved 13C-NMR spectra, especially after the removal of non-stoichiometric O-acetyl groups at C-6 of GlcNAc of the ECA repeating unit and of the lipid moiety by mild acid hydrolysis (0.01 M HCl, 100 degrees C, 10 min). Subsequent 13C-NMR studies were therefore carried out with the mild-acid-treated ECA of P. shigelloides which allowed a tentative assignment of all resonances of the ECA repeating unit. 13C-NMR spectra of Salmonella and Shigella ECA were essentially the same as those obtained with Plesiomonas ECA. The same trisaccharide repeating unit was encountered as demonstrated previously in the cyclic form of ECA isolated from S. sonnei by Dell et al. [Carbohydr. Res. 133, 95-104 (1984)]. Methylation analysis, however, afforded small amounts of terminal GlcNAc thus proving, in combination with the demonstration of the attached lipid moiety, an acyclic nature of ECA from P. shigelloides and from the two enterobacterial species. The question of whether the cyclic form co-exists in S. sonnei phase I and possibly in other enterobacterial species or, whether it had been formed during extraction as an artifact, has not yet been answered. The way in which ECA was isolated in our studies would preclude the presence of a non-amphiphilic (cyclic) polysaccharide. The finding that the sugar chain of ECA is attached to an L-glycerophosphatidyl residue is in full corroboration with serological, enzymatic and gel electrophoretic studies shown in the preceding paper and with the character of ECA as a surface antigen being anchored by hydrophobic interactions in the outer membrane of Enterobacteriaceae and P. shigelloides.
Authors:
S Basu; H M Kuhn; A Neszmelyi; K Himmelspach; H Mayer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  162     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-03-30     Completed Date:  1987-03-30     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  75-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Antigens, Bacterial / isolation & purification*
Carbohydrates / isolation & purification
Fatty Acids / analysis
Hydrogen-Ion Concentration
Hydrolysis
Magnetic Resonance Spectroscopy
Mass Spectrometry
Methylation
Vibrionaceae / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Carbohydrates; 0/Fatty Acids; 0/enterobacterial common antigen

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