Document Detail


Chemical characterization of angiotensin I in porcine follicular fluid.
MedLine Citation:
PMID:  2342987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the search for novel neuropeptides in porcine follicular fluid (pff) using smooth muscle contractile activity as a response parameter, a substance with a marked activity was isolated in a pure form. By amino acid analysis and sequential study, this substance has been chemically revealed to be angiotensin I. A much smaller amount of additional activity was isolated and found to be angiotensin II, as determined by radioimmunoassay. Radioimmunoassays for angiotensin I and II confirmed that the amount of angiotensin I determined was much greater than that of angiotensin II. A comparative study of the extractions, however, indicated a large amount of angiotensin I had been generated from angiotensinogen by endogenous renin in the follicular fluid which could be activated during extraction and ultrafiltration at a low pH. These findings are consistent with the previous reports that described a high concentration of prorenin in the follicular fluid, acid activation of prorenin to renin and the subsequent generation of angiotensin I from endogenous angiotensinogen.
Authors:
A Miyata; L J Ge; N Minamino; A Arimura
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Peptides     Volume:  11     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:    1990 Jan-Feb
Date Detail:
Created Date:  1990-06-25     Completed Date:  1990-06-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  117-21     Citation Subset:  IM    
Affiliation:
US-Japan Biomedical Research Laboratories, Tulane University Hebert Center, Bell Chasse, LA 70037.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Angiotensin I* / analysis
Animals
Biological Assay
Female
Follicular Fluid / analysis*
Guinea Pigs
Molecular Sequence Data
Muscle Contraction / drug effects
Muscle, Smooth / drug effects
Radioimmunoassay
Swine
Grant Support
ID/Acronym/Agency:
HD 6-2942/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
9041-90-1/Angiotensin I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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