Document Detail


Chemical and biological properties of B16 murine melanoma cells grown in defined medium containing bovine serum albumin.
MedLine Citation:
PMID:  144559     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The addition of 1 percent (w/v) bovine serum albumin (BSA) to the F12 medium utilized for the growth of the B16 melanoma cells significantly stimulated the growth of this cell line. The synthesis of mucopolysaccharides and sialoglycopeptides in this medium is identical with that in Eagle's minimal essential medium with Earle's balanced salt solution supplemented with 2 mM L-glutamine, twice the recommended concentration of vitamins, nonessential amino acids, sodium pyruvate, and 10 percent (v/v) fetal calf serum. Cell volume and morphology did not change significantly, under the different growth conditions and tumorigenicity, as assayed by injection of cultured cells into syngeneic animals, was not decreased. Analysis of the BSA used indicated the presence of a sialoglycoprotein contaminant. This sialoglycoprotein contaminant was present in all lots examined and contains N-acetyl-and N-glycolylneuraminic acid, mannose, galactose, and glucosamine. The sialoglycoprotein can be removed by chromatography on acetate form anion-exchange resin at pH 4.3. F12 media containing the purified BSA plus selenite and the sodium salts of palmitic, oleic, and linoleic acids supported growth of the melanoma cells to the same extent as did the media containing unpurified BSA, indicating that the sialoglycoprotein has no role in sustaining the growth of the cells.
Authors:
J R Banks; V P Bhavanadan; E A Davidson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  37     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-01-27     Completed Date:  1978-01-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4336-45     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Cell Line
Culture Media
Glycosaminoglycans / biosynthesis
Growth Substances
Kinetics
Melanoma / metabolism*,  pathology
Neoplasm Proteins / biosynthesis,  isolation & purification
Neoplasms, Experimental / metabolism
Serum Albumin, Bovine
Sialoglycoproteins / biosynthesis,  isolation & purification
Chemical
Reg. No./Substance:
0/Culture Media; 0/Glycosaminoglycans; 0/Growth Substances; 0/Neoplasm Proteins; 0/Serum Albumin, Bovine; 0/Sialoglycoproteins

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