Document Detail


Chemical binding of folic acid and methotrexate to bovine fibrinogen.
MedLine Citation:
PMID:  6685928     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The binding of folic acid as a model compound and methotrexate as a representative of antifolates to bovine fibrinogen with the aid of 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide was investigated in order to study the possibility of using fibrinogen as a drug carrier. Soluble modified fibrinogen derivatives containing 0.03-0.1 mg of folic acid or methotrexate per mg of protein were obtained under optimal conditions. These derivatives retained the ability to form fibrin clot by the action of thrombin and to copolymerize with native fibrinogen to the three dimensional fibrin network. At higher concentrations of water soluble carbodiimide, higher temperature and low pH highly cross-linked derivatives of fibrinogen and folic acid (or methotrexate) were formed which were insoluble in water and salt solutions (pseudofibrin). The modified fibrin was extensively proteolytically cleaved by plasmin, pepsin, trypsin and cathepsin D, whereas the proteolysis of insoluble pseudofibrin was very slow.
Authors:
J E Dyr; K Slavík; Z Vodrázka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Thrombosis research     Volume:  31     ISSN:  0049-3848     ISO Abbreviation:  Thromb. Res.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0326377     Medline TA:  Thromb Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  737-46     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle / blood*
Chemical Phenomena
Chemistry
Ethyldimethylaminopropyl Carbodiimide / pharmacology
Fibrinogen / metabolism*
Folic Acid / metabolism*
Methotrexate / metabolism*
Time Factors
Chemical
Reg. No./Substance:
1892-57-5/Ethyldimethylaminopropyl Carbodiimide; 59-05-2/Methotrexate; 59-30-3/Folic Acid; 9001-32-5/Fibrinogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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