| Chemical analyses of membranes isolated from Streptococcus mutans BHT. | |
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MedLine Citation:
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PMID: 6392377 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell membranes of Streptococcus mutans BHT (serotype b) were prepared following glass-bead disruption or mutanolysin digestion of whole cells. Major constituents of purified BHT membranes included: protein (60-65%), fatty acids (10%), glucose (3%), and phosphorus (0.5%). The principal amino acids measured were glutamate, aspartate, lysine, alanine, and leucine. The principal fatty acids measured were octadecenoic, palmitoleic, palmitic, and eicosenoic acids; smaller amounts of eicosanoic acid were also detected. Chemical analyses of membranes from cells grown to four different growth phases revealed no major shifts in composition during batch growth under our experimental conditions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these four membrane preparations confirmed the apparent compositional stability of cell membranes during growth. |
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Authors:
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P J Crowley; S F Hurst; A S Bleiweis |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of dental research Volume: 63 ISSN: 0022-0345 ISO Abbreviation: J. Dent. Res. Publication Date: 1984 Dec |
Date Detail:
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Created Date: 1985-02-21 Completed Date: 1985-02-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0354343 Medline TA: J Dent Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1343-7 Citation Subset: D; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Cell Membrane / analysis Electrophoresis, Polyacrylamide Gel Fatty Acids / analysis Hexoses / analysis Membrane Lipids / analysis Membrane Proteins / analysis Phosphorus / analysis Sodium Dodecyl Sulfate Streptococcus mutans / analysis, ultrastructure* Streptococcus pyogenes / ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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DE 06051/DE/NIDCR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Fatty Acids; 0/Hexoses; 0/Membrane Lipids; 0/Membrane Proteins; 151-21-3/Sodium Dodecyl Sulfate; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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