Document Detail

Chemical analyses of membranes isolated from Streptococcus mutans BHT.
MedLine Citation:
PMID:  6392377     Owner:  NLM     Status:  MEDLINE    
Cell membranes of Streptococcus mutans BHT (serotype b) were prepared following glass-bead disruption or mutanolysin digestion of whole cells. Major constituents of purified BHT membranes included: protein (60-65%), fatty acids (10%), glucose (3%), and phosphorus (0.5%). The principal amino acids measured were glutamate, aspartate, lysine, alanine, and leucine. The principal fatty acids measured were octadecenoic, palmitoleic, palmitic, and eicosenoic acids; smaller amounts of eicosanoic acid were also detected. Chemical analyses of membranes from cells grown to four different growth phases revealed no major shifts in composition during batch growth under our experimental conditions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of these four membrane preparations confirmed the apparent compositional stability of cell membranes during growth.
P J Crowley; S F Hurst; A S Bleiweis
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of dental research     Volume:  63     ISSN:  0022-0345     ISO Abbreviation:  J. Dent. Res.     Publication Date:  1984 Dec 
Date Detail:
Created Date:  1985-02-21     Completed Date:  1985-02-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0354343     Medline TA:  J Dent Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1343-7     Citation Subset:  D; IM    
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MeSH Terms
Amino Acids / analysis
Cell Membrane / analysis
Electrophoresis, Polyacrylamide Gel
Fatty Acids / analysis
Hexoses / analysis
Membrane Lipids / analysis
Membrane Proteins / analysis
Phosphorus / analysis
Sodium Dodecyl Sulfate
Streptococcus mutans / analysis,  ultrastructure*
Streptococcus pyogenes / ultrastructure
Grant Support
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids; 0/Hexoses; 0/Membrane Lipids; 0/Membrane Proteins; 151-21-3/Sodium Dodecyl Sulfate; 7723-14-0/Phosphorus

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