Document Detail

Chelation of GRP78 with lead and its localization changes in the astroglia of rats exposed to lead.
MedLine Citation:
PMID:  19662369     Owner:  NLM     Status:  In-Process    
To observe the chelation of GRP78 with lead (Pb) and its localization changes, astroglial cells from Wistar rat brain were primarily cultured in medium with acetate Pb. The processes were terminated at different time points. The immunoprecipitation (IP) and Western blotting were used for GRP78 purification and expression and the Pb concentration was determined by employing atomic absorption spectrophotometry (AAS). The localization change of GRP78 was observed with colloid gold immunoelectron microscopy. The results showed that the expression of GRP78 was increased significantly in the cells treated with 1.0 micromol/L acetate Pb for 24 h and peaked at 96-192 h (P<0.01), and at the 12th day, the expression of GRP78 began to decrease but was still higher than normal (P<0.05). Pb content started to increase when cells were treated by acetate Pb for 24 h, and the peak appeared at 8 day (P<0.01), and then Pb content decreased gradually, but was still higher than normal (P<0.05). GRP78 protein expression began to remarkably increase when it transferred from ER to the cytosol around the nuclei 24 h after treatment with Pb. It is concluded that GRP78 in astroglia could strongly chelate with Pb ions and it might be a target protein of Pb.
Ying Zhang; Liping Ye; Biao Wang; Yan Li; Liguang Sun
Related Documents :
913919 - Barbiturate-induced choleresis: possible independence from microsomal enzyme induction.
870909 - Phenobarbital drinking curves after varied water deprivation.
20732159 - Effect of metabolism on the anticholinesterase activity of parathion and paraoxon in pr...
25104279 - Animal allergen sensitization in veterinarians and laboratory animal workers.
7450889 - Monooxygenase activity and ultrastructural changes of liver in the course of chronic ex...
25323289 - Histomorphometric evaluation of maxillary molar roots and surrounding periodontium foll...
8388459 - Adrenergic, serotonergic and cholinergic components of nicotinic antinociception in rats.
3980359 - Transient depletion of lung glutathione by diethylmaleate enhances oxygen toxicity.
25036159 - Effects of beraprost sodium on renal function and inflammatory factors of rats with dia...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-07
Journal Detail:
Title:  Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban     Volume:  29     ISSN:  1672-0733     ISO Abbreviation:  J. Huazhong Univ. Sci. Technol. Med. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101169627     Medline TA:  J Huazhong Univ Sci Technolog Med Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  492-7     Citation Subset:  IM    
Institute of Neurology, China Medical University, Shenyang, 110001, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Bacterial translocation and change in intestinal permeability in patients after abdominal surgery.
Next Document:  Effects of 5-Aza-CdR on the proliferation of human breast cancer cell line MCF-7 and on the expressi...