Document Detail


Charge-driven selective localization of fluorescent nanoparticles in live cells.
MedLine Citation:
PMID:  22796738     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Covalent grafting of amino groups onto the carboxylic acid functionalities, naturally covering the surface of fluorescent nanoparticles produced from silicon carbide (SiC NPs), allowed tuning of their surface charge from negative to highly positive. Incubating 3T3-L1 fibroblast cells with differently charged SiC NPs demonstrates the crucial role of the charge in cell fluorescent targeting. Negatively charged SiC NPs concentrate inside the cell nuclei. Close to neutrally charged SiC NPs are present in both cytoplasm and nuclei while positively charged SiC NPs are present only in the cytoplasm and are not able to move inside the nuclei. This effect opens the door for the use of SiC NPs for easy and fast visualization of long-lasting biological processes taking place in the cell cytosol or nucleus as well as providing a new long-term cell imaging tool. Moreover, here we have shown that the interaction between charged NPs and nuclear pore complex plays an essential role in their penetration into the nuclei.
Authors:
T Serdiuk; S A Alekseev; V Lysenko; V A Skryshevsky; A Géloën
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Publication Detail:
Type:  Journal Article     Date:  2012-07-13
Journal Detail:
Title:  Nanotechnology     Volume:  23     ISSN:  1361-6528     ISO Abbreviation:  Nanotechnology     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101241272     Medline TA:  Nanotechnology     Country:  England    
Other Details:
Languages:  eng     Pagination:  315101     Citation Subset:  IM    
Affiliation:
University of Lyon, CarMeN Laboratory, INSA de Lyon, UMR INSERM 1060, France. Institute of High Technologies, Taras Shevchenko National University of Kyiv, 64 Volodymyrs'ka, 01601 Kyiv, Ukraine.
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