Document Detail

Characterizing stearate probes in vitro for the electrochemical detection of dopamine and serotonin.
MedLine Citation:
PMID:  2776030     Owner:  NLM     Status:  MEDLINE    
This paper describes an electrochemical method for the detection of dopamine and serotonin in vitro. Dopamine and serotonin can be distinguished from each other without interference from metabolites. 3-4-dihydroxyphenylacetic acid and 5-hydroxyindoleacetic acid. The method also provides selective differentiation of dopamine and serotonin in the presence of other possible interfering chemicals, ascorbic acid and uric acid. Specific details for voltammetric technology, electrode fabrication, electrode conditioning, paste synthesis and experimental protocol are presented. The method uses semidifferential treatment of voltammetric data in conjunction with a graphite stearate indicator electrode. The methodology is relevant to the interpretation of electrochemical signals for dopamine and serotonin in vivo in neuroanatomical substrates, richly innervated by dopaminergic and serotonergic neuronal circuitry.
P A Broderick
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  495     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1989 Aug 
Date Detail:
Created Date:  1989-10-19     Completed Date:  1989-10-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  115-21     Citation Subset:  IM    
Department of Pharmacology, City University of New York Medical School, NY 10031.
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MeSH Terms
Dopamine / analysis*
Electrochemistry / instrumentation,  methods*
Serotonin / analysis*
Grant Support
2-S07-RR07132/RR/NCRR NIH HHS; 20541//PHS HHS
Reg. No./Substance:

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