Document Detail

Characterizing the roles of Met31 and Met32 in coordinating Met4-activated transcription in the absence of Met30.
MedLine Citation:
PMID:  22438580     Owner:  NLM     Status:  MEDLINE    
Yeast sulfur metabolism is transcriptionally regulated by the activator Met4. Met4 lacks DNA-binding ability and relies on interactions with Met31 and Met32, paralogous proteins that bind the same cis-regulatory element, to activate its targets. Although Met31 and Met32 are redundant for growth in the absence of methionine, studies indicate that Met32 has a prominent role over Met31 when Met30, a negative regulator of Met4 and Met32, is inactive. To characterize different roles of Met31 and Met32 in coordinating Met4-activated transcription, we examined transcription in strains lacking either Met31 or Met32 upon Met4 induction in the absence of Met30. Microarray analysis revealed that transcripts involved in sulfate assimilation and sulfonate metabolism were dramatically decreased in met32Δ cells compared to its wild-type and met31Δ counterparts. Despite this difference, both met31Δ and met32Δ cells used inorganic sulfur compounds and sulfonates as sole sulfur sources in minimal media when Met30 was present. This discrepancy may be explained by differential binding of Met31 to Cbf1-dependent promoters between these two conditions. In the absence of Met30, genome-wide chromatin immunoprecipitation analyses found that Met32 bound all Met4-bound targets, supporting Met32 as the main platform for Met4 recruitment. Finally, Met31 and Met32 levels were differentially regulated, with Met32 levels mimicking the profile for active Met4. These different properties of Met32 likely contribute to its prominent role in Met4-activated transcription when Met30 is absent.
Emilio Carrillo; Giora Ben-Ari; Jan Wildenhain; Mike Tyers; Dilon Grammentz; Traci A Lee
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-21
Journal Detail:
Title:  Molecular biology of the cell     Volume:  23     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-14     Completed Date:  2012-09-10     Revised Date:  2014-02-24    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1928-42     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Basic-Leucine Zipper Transcription Factors / metabolism*
Binding Sites
Cluster Analysis
Consensus Sequence
DNA-Binding Proteins / genetics,  metabolism,  physiology*
F-Box Proteins / genetics
Gene Expression Profiling
Gene Expression Regulation, Fungal
Oligonucleotide Array Sequence Analysis
Promoter Regions, Genetic
Protein Binding
Saccharomyces cerevisiae / genetics*,  growth & development,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*,  physiology*
Sulfonic Acids / metabolism
Transcription Factors / genetics,  metabolism,  physiology*
Transcription, Genetic
Transcriptional Activation*
Ubiquitin-Protein Ligase Complexes / deficiency*,  genetics
Grant Support
092076//Wellcome Trust; R15GM086847/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Basic-Leucine Zipper Transcription Factors; 0/DNA-Binding Proteins; 0/F-Box Proteins; 0/MET4 protein, S cerevisiae; 0/Met31 protein, S cerevisiae; 0/Met32 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Sulfonic Acids; 0/Transcription Factors; EC protein, S cerevisiae; EC Ligase Complexes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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