Document Detail


Characterizing the effects of aligned collagen fibers and ascorbic acid derivatives on behavior of rabbit corneal fibroblasts.
MedLine Citation:
PMID:  19963815     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cornea is responsible for functional optical activity of the mammalian eye, as it must remain transparent in order to focus light onto the retina. Corneal disease is the second leading cause worldwide of vision loss [1]. Human donor tissue transplantation in the cornea is associated with problems such as immunorejection and recurring graft failures [1]. Tissue engineering offers a promising alternative to using human donor tissues in treating corneal diseases. A viable tissue-engineered cornea must be mechanically resilient to protect the fragile intraocular components of the eye, and optically transparent to refract light onto the retina. In the native cornea, transparency is maintained by both the cells in the stromal layer and the high organization of the extracellular matrix (ECM). This study aims to combine the effects of aligned collagen fibers and ascorbic acid derivatives to control corneal fibroblast behavior to not only express the appropriate proteins, but also to deposit aligned, small diameter collagen fibers that resemble the highly organized structure of the natural ECM. Results from this study suggest that the combined effect of an aligned scaffolding material and ascorbic acid supplements can create a cell-matrix construct that both downregulates expression of the light scattering protein a-smooth muscle actin (alpha-sma) and supports an increased number of cell layers.
Authors:
Donna Phu; Elizabeth J Orwin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference     Volume:  2009     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2009  
Date Detail:
Created Date:  2009-12-07     Completed Date:  2010-04-22     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4242-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Animals
Ascorbic Acid / metabolism*
Cells, Cultured
Collagen / chemistry*
Cornea / pathology*
Culture Media / metabolism
Equipment Design
Fibroblasts / metabolism,  pathology*
Microscopy, Confocal / methods
Microscopy, Electron, Scanning / methods
Muscle, Smooth / metabolism
Rabbits
Retina / metabolism
Tissue Engineering / methods*
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Actins; 0/Culture Media; 9007-34-5/Collagen; PQ6CK8PD0R/Ascorbic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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