Document Detail


Characterizing the effects of aligned collagen fibers and ascorbic acid derivatives on behavior of rabbit corneal fibroblasts.
MedLine Citation:
PMID:  19963815     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The cornea is responsible for functional optical activity of the mammalian eye, as it must remain transparent in order to focus light onto the retina. Corneal disease is the second leading cause worldwide of vision loss [1]. Human donor tissue transplantation in the cornea is associated with problems such as immunorejection and recurring graft failures [1]. Tissue engineering offers a promising alternative to using human donor tissues in treating corneal diseases. A viable tissue-engineered cornea must be mechanically resilient to protect the fragile intraocular components of the eye, and optically transparent to refract light onto the retina. In the native cornea, transparency is maintained by both the cells in the stromal layer and the high organization of the extracellular matrix (ECM). This study aims to combine the effects of aligned collagen fibers and ascorbic acid derivatives to control corneal fibroblast behavior to not only express the appropriate proteins, but also to deposit aligned, small diameter collagen fibers that resemble the highly organized structure of the natural ECM. Results from this study suggest that the combined effect of an aligned scaffolding material and ascorbic acid supplements can create a cell-matrix construct that both downregulates expression of the light scattering protein a-smooth muscle actin (alpha-sma) and supports an increased number of cell layers.
Authors:
Donna Phu; Elizabeth J Orwin
Related Documents :
1122095 - Some biochemical aspects of light effects on transparent eye tissues.
10676075 - Circumscribed posterior keratoconus: case report.
3309765 - Treatment of mooren's and mooren's-like ulcer by lamellar keratectomy: report of six ey...
12792485 - Bilateral corneal opacities associated with idiopathic multicentric osteolysis.
24647595 - One-year refractive results, contrast sensitivity, high-order aberrations and complicat...
24148655 - Sutureless 27-gauge needle-guided intrascleral intraocular lens implantation with lamel...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference     Volume:  1     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2009  
Date Detail:
Created Date:  2009-12-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4242-5     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Duke University, Durham, NC 27705 USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  SIMENGINE: A low-cost, high-performance platform for embedded biophysical simulations.
Next Document:  Thermoresponsive hydrogel with embedded magnetic nanoparticles for the implementation of shrinkable ...