| Characterizing Vaccinium berry Standard Reference Materials by GC-MS using NIST spectral libraries. | |
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MedLine Citation:
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PMID: 23291961 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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A gas chromatography-mass spectrometry (GC-MS)-based method was developed for qualitative characterization of metabolites found in Vaccinium fruit (berry) dietary supplement Standard Reference Materials (SRMs). Definitive identifications are provided for 98 unique metabolites determined among six Vaccinium-related SRMs. Metabolites were enriched using an organic liquid/liquid extraction, and derivatized prior to GC-MS analysis. Electron ionization (EI) fragmentation spectra were searched against EI spectra of authentic standards compiled in the National Institute of Standards and Technology's mass spectral libraries, as well as spectra selected from the literature. Metabolite identifications were further validated using a retention index match along with prior probabilities and were compared with results obtained in a previous effort using collision-induced dissociation (CID) MS/MS datasets from liquid chromatography coupled to mass spectrometry experiments. This manuscript describes a nontargeted metabolite profile of Vaccinium materials, compares results among related materials and from orthogonal experimental platforms, and discusses the feasibility and development of using mass spectral library matching for nontargeted metabolite identification. |
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Authors:
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Mark S Lowenthal; Nirina R Andriamaharavo; Stephen E Stein; Karen W Phinney |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-6 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: - ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-7 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Biomolecular Measurement Division, National Institute of Standards and Technology, 100 Bureau Drive, Stop 8392, Gaithersburg, MD, 20899-8392, USA, mark.lowenthal@nist.gov. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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