Document Detail

Characterization of the weak calcium binding of trimeric globular adiponectin.
MedLine Citation:
PMID:  23023478     Owner:  NLM     Status:  Publisher    
Adiponectin is secreted from adipose tissue and functions as a protein hormone in regulating glucose metabolism and fatty acid catabolism. Adiponectin plays an important role as a novel risk factor and potential diagnostic and prognostic biomarker in cancer. Crystal structures of globular adiponectin have been resolved with three calcium-binding sites on the top of its central tunnel. However, the calcium-binding property of adiponectin remains elusive. Mouse globular adiponectin was cloned into pET11a and expressed in Escherichia coli. The folding of adiponectin was indicated by the spread of resonances in HSQC spectrum. Luminescence resonance energy transfer was used to obtain the binding constant (K(d) ) of Tb(3+) and the inhibitor constant (K(i) ) of Ca(2+) for globular adiponectin. The obtained calcium-binding affinity to adiponectin is relatively low (~2 mM), which indicates that the high concentration of adiponectin in circulating system may function as calcium storage bank and buffer the free calcium concentration. Copyright © 2012 John Wiley & Sons, Ltd.
Dongmei Yu; Chao Zhang; Han Wang; Peiwu Qin
Related Documents :
11985468 - Synthesis, in vitro receptor binding, and in vivo evaluation of fluorescein and carbocy...
7735688 - Structural requirements at the melatonin receptor.
1470308 - Benextramine-neuropeptide y (npy) binding site interactions: characterization of 3h-npy...
8156108 - Cortisol decreases 2[125i]iodomelatonin binding sites in the duck thymus.
11765148 - Studies on receptor binding and signal transduction pathways of unoprostone isopropyl.
6601608 - Conformational changes of 4-carboxyglutamic acid-containing protein from bovine bone by...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-1
Journal Detail:
Title:  Cell biochemistry and function     Volume:  -     ISSN:  1099-0844     ISO Abbreviation:  Cell Biochem. Funct.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8305874     Medline TA:  Cell Biochem Funct     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 John Wiley & Sons, Ltd.
Department of Biological Engineering, University of Missouri, Columbia, MO, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Who Should Be Reading Chest Radiographs in the Pediatric Emergency Department?
Next Document:  Oxovanadium(iv)-catalysed oxidation of dibenzothiophene and 4,6-dimethyldibenzothiophene.